|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-RSK1 p90 Antibody|
|Description||Rabbit IgG polyclonal antibody for Ribosomal protein S6 kinase alpha-1(RPS6KA1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-RSK1 p90 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2052)|
|Replacement Item||This antibody may replace the following items: sc-101770|sc-11756|sc-11756-R|sc-12883|sc-12883-R|sc-130870|sc-135634|sc-136476|sc-17033-R|sc-231|sc-231-G|sc-28773|sc-377526|sc-393147|sc-74575|sc-81162|sc-81758 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human RSK1 p90(721-735aa ILAQRRVRKLPSTTL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ribosomal protein S6 kinase alpha-1|
|Molecular Weight||82723 MW|
|Protein Function||Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin- derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.|
|Subcellular Localization||Nucleus. Cytoplasm.|
|Alternative Names||Ribosomal protein S6 kinase alpha-1;S6K-alpha-1;126.96.36.199;90 kDa ribosomal protein S6 kinase 1;p90-RSK 1;p90RSK1;p90S6K;MAP kinase-activated protein kinase 1a;MAPK-activated protein kinase 1a;MAPKAP kinase 1a;MAPKAPK-1a;Ribosomal S6 kinase 1;RSK-1;RPS6KA1;MAPKAPK1A, RSK1;|
|Research Areas|||signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| epigenetics and nuclear signaling|transcription|other factors||
Background for Ribosomal protein S6 kinase alpha-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-RSK1 p90 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-RSK1 p90 Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: K562 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: SW620 Cell Lysate
Lane 6: RAJI Cell Lysate
All lanes: Anti-RPS6KA1 (PA2052) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,