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MTOR antibody (P00003) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 37 Publications

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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-mTOR (S2448) Antibody.

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Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody

Name: Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: P00003
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

MTOR antibody

37 Publications|

2 Reviews

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IHC, WB

Tested Applications

MTOR antibody was positively detected by WB in:

HEK293 cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MTOR antibody was positively detected by IHC in:

human breast cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Product Information

Product Name

Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
View all MTOR Antibodies

Form

Liquid

Uniprot

P42345

Gene ID

2475

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

IFF-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-mTOR (S2448)

Observed Molecular Weight

289 kDa

Calculated Molecular Weight

288.9 kDa

Cite This Product

Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00003)

Protocols

Western Blot protocol for MTOR antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MTOR antibody

Review Protocol

Publications

Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation

Published: 2026-05-11

Journal: MATERIALS & DESIGN

Application: WB

Impact Factor: 8.20

Species: Rat

SKU	P00003
Application	Western Blot
Sample	mouse 4T1 cells
Sample Processing Description	normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation	1:2500, overnight at 4 ℃
Secondary Antibody	HRP Goat Anti-Rabbit IgG
Secondary Incubation	1:10000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	With equal loading amounts, total MTOR protein levels were similar across all groups. However, phosphorylated MTOR levels showed significant changes: the blank group was the lowest, the model group the highest, and in the drug-treated groups, phosphorylation decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

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Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody

MTOR antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation

YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis

BEZ235 enhances the photodynamic antitumor efficacy of hypoxia-responsive BODIPY prodrug nanoparticles by inhibiting the PI3K/mTOR pathway and alleviating hypoxia

Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

Exploring the interaction mechanisms of CD46/TREM1 and LC3B/ATG5 in the inflammation-cancer transformation of oral squamous cell carcinoma based on bioinformatics

Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment

Function of AMPK/mTOR Signaling in TGF-β1-Induced Pterygium Fibroblast Proliferation and Transdifferentiation

1,25(OH)2D3 induces chondrocyte autophagy and reduces the loss of proteoglycans in osteoarthritis through inhibiting the NF-κB pathway

Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro

The mechanism of blood coagulation induced by sodium dehydroacetate via the regulation of the mTOR/ERK pathway in rats

The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma

Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland

Connexin 43-modified bone marrow stromal cells reverse the imatinib resistance of K562 cells via Ca2+-dependent gap junction intercellular communication

Effects of lactate on metabolism and differentiation of CD4+T cells

Moxidectin induces autophagy arrest in colorectal cancer

The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo

Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling

Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo

Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway

Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells

Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway

Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells

Moxidectin induces Cytostatic Autophagic Cell Death of Glioma Cells through inhibiting the AKT/mTOR Signalling Pathway

Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells

MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1

CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells

MiR-29 regulates the function of goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways

A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development

miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways

Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles

Ivermectin induces autophagy-mediated cell death through the AKT/mTOR signaling pathway in glioma cells

ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro

Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling

Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

Circ-8073 regulates CEP55 by sponging miR-449a to promote caprine endometrial epithelial cells proliferation via the PI3K/AKT/mTOR pathway

Rapamycin provides anti‑epileptogenic effect in a rat model of post‑traumatic epilepsy via deactivation of mTOR signaling pathway

Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway

Review

Have you used Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody?

2 Reviews For Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody

The antibody shows a clear target band, correct localization, minimal background, and overall good results.

This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has well-defined edges.

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