Anti-Rubicon RUBCN Antibody

Product Name

Anti-Rubicon RUBCN Antibody

View all KIAA0226 Antibodies

SKU/Catalog Number

A12453

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Rubicon RUBCN Antibody (Catalog # A12453). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Rubicon RUBCN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12453)

Host

Rabbit

Contents

Rubicon antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.

Cross-reactivity

Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.

Reactive Species

A12453 is reactive to KIAA0226 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

108.6 kDa

Background of KIAA0226

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12453 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information KIAA0226

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