Click image to see more details
Product Info Summary
| SKU: | A00155 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-RyR-2 Antibody
SKU/Catalog Number
A00155
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RyR-2 Antibody catalog # A00155. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RyR-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00155)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RyR2. AA range:2774-2823
Reactive Species
A00155 is reactive to RYR2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
564.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00155 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of HepG2-UV MOUSE-BRAIN AD293 PC-3 cells using RyR-2 Polyclonal Antibody diluted at 1:2000
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RyR2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RyR-2 Antibody (A00155)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-RyR-2 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum hippocampus using anti-RyR-2 antibody A00155. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-15
Question
Is a blocking peptide available for product anti-RyR-2 antibody (A00155)?
D. Jha
Verified customer
Asked: 2018-02-16
Answer
We do provide the blocking peptide for product anti-RyR-2 antibody (A00155). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-16
Question
Do you have a BSA free version of anti-RyR-2 antibody A00155 available?
N. Parker
Verified customer
Asked: 2018-01-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RyR-2 antibody A00155 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-29
Question
I have a question about product A00155, anti-RyR-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Krishna
Verified customer
Asked: 2017-10-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00155 anti-RyR-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-23
Question
We are currently using anti-RyR-2 antibody A00155 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
J. Wu
Verified customer
Asked: 2014-03-17
Answer
The anti-RyR-2 antibody (A00155) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-17
