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- Table of Contents
S100a9 antibody (PB9678) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of HEPA1-6 cells using anti-S100A9 antibody (PB9678).
Overlay histogram showing HEPA1-6 cells stained with PB9678 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A9 Antibody (PB9678, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with PB9678 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A9 Antibody (PB9678, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for S100A9? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the S100A9 Western Blot GuideBundle Save
Anti-S100A9 Antibody Picoband®
S100a9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
S100a9 antibody was positively detected by WB in:
mouse spleen tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
S100a9 antibody was positively detected by IHC in:
mouse spleen tissue, rat spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
S100a9 antibody was positively detected by ICC/IF in:
HEPA1-6 cell
S100a9 antibody was positively detected by FC in:
HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-S100A9 Antibody Picoband®
View all S100A9 Antibodies
View all S100A9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.
Cross-Reactivity
Cross-reactivity with recombinant human S100A9 is observed.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
13-15 kDa
Calculated Molecular Weight
13.0 kDa
Background of S100a9
S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.
Cite This Product
Anti-S100A9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9678)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
79%
Moderate Homology
Rabbit
60%
Low homology
Cow
60%
Low homology
Monkey
60%
Low homology
Human
58%
Low homology
Sheep
54%
Low homology
Dog
54%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for S100a9 antibody
Immunohistochemistry (IHC) protocol for S100a9 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for S100a9 antibody
Flow Cytometry (FC) protocol for S100a9 antibody
Publications
Application:
Species:
Myricetin Attenuates IMQ-Induced Psoriatic Inflammation Through Multi-Target Modulation: Evidence from Network Pharmacology and Experimental Validation
Published: 2025-11-26
Journal: Pharmaceuticals
Application: IHC
Impact Factor: 4.80
Species: Mouse
Harnessing Distinct Tissue-Resident Immune Niches via S100A9/TLR4 Improves Ketone, Lipid, and Glucose Metabolism
Published: 2025-08-28
Journal: ENDOCRINOLOGY
Application: ELISA,WB
Impact Factor: 3.30
Species: Mouse
PMID: 40874857
Neutrophil Membrane-Engineered Extracellular Vesicles from Perivascular Adipose Tissue-Derived Stromal Cells for Atherosclerotic Lesion Resolution and Enhanced Vascular Graft Remodeling
Published: 2025-06-02
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: IHC
Impact Factor: 19.00
Species: Rat
Pulmonary mitochondrial DNA release and activation of the cGAS-STING pathway in Lethal Stx12 knockout mice
Published: 2025-04-08
Journal: Cell Communication and Signaling
Application: WB,IF
Impact Factor: 8.90
Species: Mouse
PMID: 40200300
Mechanism of S100A9-mediated astrocyte activation via TLR4/NF-κB in Parkinson’s disease
Published: 2024-12-25
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB,IF
Impact Factor: 4.80
Species: Mouse
PMID: 39724736
Single-cell RNA sequencing of estrual mice reveals PM2.5-induced uterine cell heterogeneity and reproductive toxicity
Published: 2024-09-04
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: IF
Impact Factor: 6.20
Species: Mouse
PMID: 39236655
Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats
Published: 2017-06-20
Journal: Oncotarget
Application: WB
Impact Factor: 5.17
Species: Rat
PMID: 28881824
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Product Promise
We promise Anti-S100A9 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9678
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026