Human MyD88 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MyD88. 96wells/kit, with removable strips.
Human MyD88 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, bone tissue, serum and plasma (heparin, EDTA)|
|Sample Volume:||100μl per well|
|Product Name||Human MyD88 ELISA Kit PicoKine™
See all MYD88 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human MYD88 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, bone tissue, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MYD88 tissue specificity: Ubiquitous. .|
|Cite This Product||Human MyD88 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0942)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, bone tissue, serum and plasma (heparin, EDTA)|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: M1-P296|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For MYD88 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4615|
|Species Of This Entry||Human|
|Protein Name||Myeloid differentiation primary response protein MyD88|
|Alternative Names||MYD88|MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Ubiquitination; Glycosylation; Phosphorothioation; Acylation; Acetylation; Oxidation; Dephosphorylation; Ribosylation; Biotinylation; Nitration; Sulphation; Nitrosylation; Citrullination; Gpi Anchoring; Farnesylation; Deglycosylation; Myristoylation|
|Subcellular Localization||Cytoplasm. Nucleus.|
|Gene Location||3p22.2, on Chromasome 3, gene sequence: NC_000003.12 (38138661..38143022)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MYD88 (Source: Uniprot.org, NCBI)
|Protein Function||Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:24316379, PubMed:19506249). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).|
|Interacting Proteins||CYTH2; DOCK8; FADD; HDAC6; IRAK1; IRAK4; SARM1; SIAH1; SMAD3; SPOP; STAP2; TIRAP; TLR2; TLR4; TNFRSF13B; TXN; USP7|
|Related Diseases||Inflammation; Innate Immune Response; Infective Disorder; Inflammatory Response; Bacterial Infections; Pneumonia; Neoplasms; Systemic Infection; Virus Diseases; Malignant Neoplasms; Autoimmune Reaction; Tuberculosis; Colitis; Autoimmune Diseases; Salmonella Infections; Cell Invasion; Autoimmunity; Fibrosis; Tissue Adhesions; Influenza|
|Related Pathways||Immune Response; Secretion; Cytokine Production; Innate Immune Response; Pathogenesis; Inflammatory Response; Cell Activation; Cell Death; Cell Proliferation; Virulence; Phagocytosis; Adaptive Immune Response; Macrophage Activation; Localization; Cytokine Secretion; Rna Interference; Response To Lipopolysaccharide; Chemokine Production; Cell Differentiation; T Cell Proliferation|
|Background||Myeloid differentiation primary response gene(88)(MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9; the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.|
|Scientific References||PMID: 8957090 by Hardiman G., et al. Molecular characterization and modular analysis of human MyD88.|
PMID: 9013863 by Bonnert T.P., et al. The cloning and characterization of human MyD88: a member of an IL-1 receptor related family.
Kit Components And Assay QC Data Details Of Human MyD88 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0942-CAP||96-well plate precoated with anti-Human MYD88 antibody||1|
|EK0942-ST||lyophilized recombinant Human MYD88 standard||10ng/tube|
|EK0942-DA||biotinylated anti-Human MYD88 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0942 Human MyD88 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human MyD88 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human MyD88 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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