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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Mouse ERAP1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse ERAP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Erap1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ERAP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2028)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M1-L930
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2028 is reactive to Erap1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK2028 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Aminopeptidase PILS/ARTS1
Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, also known as endoplasmic reticulum aminopeptidase 1 (ARTS-1), is a protein which in humans is encoded by the ARTS-1 gene. This gene is mapped to 13; 13 C1. Endoplasmic reticulum amino peptidase 1 is active in the endoplasmic reticulum, which is involved in protein processing and transport. This protein is an aminopeptidase, which is an enzyme that cleaves peptides at the N-terminal into smaller fragments called amino acids.
|EK2028-CAP||Anti-Mouse Erap1 Pre-coated 96-well strip microplate||1|
|EK2028-ST||Mouse Erap1 Standard||2 vials, 20 ng/tube|
|EK2028-DA||Mouse Erap1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse ERAP1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Aminopeptidase PILS/ARTS1, Dilution ratio of 1:100, concentration in serum is 0.25-1.3ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ERAP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Aminopeptidase PILS/ARTS1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Erap1 (Source: Uniprot.Org, NCBI)
Endoplasmic reticulum aminopeptidase 1
peptidase M1 family
Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 22.214.171.124; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator Erap1|A-LAP, ARTS-1, Ar, Arts1, E, ERAAP, PILSA, PILSAP|endoplasmic reticulum aminopeptidase 1|endoplasmic reticulum aminopeptidase 1|VEGF-induced aminopeptidase|adipocyte-derived leucine aminopeptidase|aminopeptidase PILS|puromycin-insensitive leucyl-specific aminopeptidase|type 1 tumor necrosis factor receptor shedding aminopeptidase regulator*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Erap1, check out the Erap1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Erap1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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