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Product Info Summary
| SKU: | M03816-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11)
SKU/Catalog Number
M03816-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) catalog # M03816-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03816-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
5H11
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human SAE2/UBA2 recombinant protein (Position: E449-K564).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03816-1 is reactive to UBA2 in Human, Mouse, Rat
Observed Molecular Weight
90 kDa
Calculated molecular weight
71.2 kDa
Background of UBA2
Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03816-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human K562 whole cell, human Raji whole cell, human THP-1 whole cell, human SW579 whole cell, human HepG2 whole cell, human CCRF-CEM whole cell, rat PC-12 whole cell, mouse RAW2467 whole cell,
FCM: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of UBA2 using anti-UBA2 antibody (M03816-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates
Lane 2: human Raji whole cell lysates
Lane 3: human THP-1 whole cell lysates
Lane 4: human SW579 whole cell lysates
Lane 5: human HepG2 whole cell lysates
Lane 6: human CCRF-CEM whole cell lysates
Lane 7: rat PC-12 whole cell lysates
Lane 8: mouse RAW246.7 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-UBA2 antigen affinity purified monoclonal antibody (Catalog # M03816-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for UBA2 at approximately 90KD. The expected band size for UBA2 is at 71KD.
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Flow Cytometry analysis of A431 cells using anti-UBA2 antibody (M03816-1).
Overlay histogram showing A431 cells stained with M03816-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-UBA2 Antibody (M03816-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) (M03816-1)
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Customer Q&As
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1 Customer Q&As for Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11)
Question
We are currently using anti-SAE2/UBA2 antibody (monoclonal, 5H11) M03816-1 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
K. Yang
Verified customer
Asked: 2015-02-17
Answer
The anti-SAE2/UBA2 antibody (monoclonal, 5H11) (M03816-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-17
