Overview
| Product Name |
Anti-SAE2 / UBA2 Monoclonal Antibody
See more SAE2 products |
| Catalog# |
M03816 |
| Pack Size |
100ug |
| Storage & Handling |
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Description |
Rabbit IgG Monoclonal antibody for Q9UBT2 detection. Tested positive for WB IHC IF/IF IP in Human Mouse Rat |
| Cite This Product |
Anti-SAE2 / UBA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03816)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
IP, IF, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
Gene/Protein Basic Information For UBA2 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9UBT2 |
|---|
| NCBI Gene Id | 10054 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | UBA2 |
|---|
| Protein Name | SUMO-activating enzyme subunit 2 |
|---|
| Superfamily | ubiquitin-activating E1 family |
|---|
| Alternative Names | SAE2|Anthracycline-associated resistance ARX; ARX; EC 6.3.2; EC 6.3.2.-; FLJ13058; HRIHFB2115; SAE2SUMO-1 activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2, ubiquitin-activating enzyme E1 homolog; Ubiquitin-like 1-activating enzyme E1B; ubiquitin-like modifier activating enzyme 2; UBLE1B |
|---|
| Molecular Weight | 71224 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on UBA2, check out the UBA2 Infographic
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Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for UBA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the UBA2 infographic
Our Boster Quality Guarantee for Anti-SAE2 / UBA2 Monoclonal Antibody covers its use in the following applications.
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of UBA2 expression in A549 cell lysate using anti-UBA2 antibody (M03816).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA2 antigen affinity purified polyclonal antibody (Catalog # M03816) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for UBA2.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Total number of citations: 0
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Questions and answers from customers related to M03816 Anti-SAE2 / UBA2 Monoclonal Antibody
4 Related Questions
Question
Do you have a BSA free version of anti-SAE2 / UBA2 Monoclonal antibody M03816 available?
Verified Customer
Asked: 2019-05-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SAE2 / UBA2 Monoclonal antibody M03816 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
We want to test anti-SAE2 / UBA2 Monoclonal antibody M03816 on human fetal brain for research purposes, then I may be interested in using anti-SAE2 / UBA2 Monoclonal antibody M03816 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Mangal
Asked: 2019-05-27
Answer
The products we sell, including anti-SAE2 / UBA2 Monoclonal antibody M03816, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-27
Question
Here is the WB image, lot number and protocol we used for fetal brain using anti-SAE2 / UBA2 Monoclonal antibody M03816. Please let me know if you require anything else.
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-04
Question
Would anti-SAE2 / UBA2 Monoclonal antibody M03816 work for IF with fetal brain?
J. Anderson
Asked: 2015-10-20
Answer
According to the expression profile of fetal brain, UBA2 is highly expressed in fetal brain. So, it is likely that anti-SAE2 / UBA2 Monoclonal antibody M03816 will work for IF with fetal brain.
Boster Scientific Support
Answered: 2015-10-20
