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Product Info Summary
| SKU: | M03816 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SAE2 / UBA2 Monoclonal Antibody
SKU/Catalog Number
M03816
BM5495 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SAE2 / UBA2 Monoclonal Antibody catalog # M03816. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SAE2 / UBA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03816)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEAI-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Reactive Species
M03816 is reactive to UBA2 in Human, Mouse, Rat
Observed Molecular Weight
90 kDa
Calculated molecular weight
71.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03816 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Positive Control
WB: A549 cell
Validation Images & Assay Conditions
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Western blot analysis of SAE2 / UBA2 expression in A549 cell lysate.
Specific Publications For Anti-SAE2 / UBA2 Monoclonal Antibody (M03816)
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Customer Q&As
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4 Customer Q&As for Anti-SAE2 / UBA2 Monoclonal Antibody
Question
Do you have a BSA free version of anti-SAE2 / UBA2 Monoclonal antibody M03816 available?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SAE2 / UBA2 Monoclonal antibody M03816 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
We want to test anti-SAE2 / UBA2 Monoclonal antibody M03816 on human fetal brain for research purposes, then I may be interested in using anti-SAE2 / UBA2 Monoclonal antibody M03816 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Mangal
Verified customer
Asked: 2019-05-27
Answer
The products we sell, including anti-SAE2 / UBA2 Monoclonal antibody M03816, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-27
Question
Here is the WB image, lot number and protocol we used for fetal brain using anti-SAE2 / UBA2 Monoclonal antibody M03816. Please let me know if you require anything else.
M. Jha
Verified customer
Asked: 2015-12-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-04
Question
Would anti-SAE2 / UBA2 Monoclonal antibody M03816 work for IF with fetal brain?
J. Anderson
Verified customer
Asked: 2015-10-20
Answer
According to the expression profile of fetal brain, UBA2 is highly expressed in fetal brain. So, it is likely that anti-SAE2 / UBA2 Monoclonal antibody M03816 will work for IF with fetal brain.
Boster Scientific Support
Answered: 2015-10-20
