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Product Info Summary
| SKU: | M32769 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-SCD1 Antibody (Monoclonal, 31S66)
SKU/Catalog Number
M32769
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SCD1 Antibody (Monoclonal, 31S66) catalog # M32769. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCD1 Antibody (Monoclonal, 31S66) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32769)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31S66
Immunogen
Synthetic peptide within mouse SCD1 aa 3-48.
Reactive Species
M32769 is reactive to Scd1 in Mouse, Rat
Calculated molecular weight
41.0 kDa
Background of Scd1
Enables metal ion binding activity; palmitoyl-CoA 9-desaturase activity; and stearoyl-CoA 9-desaturase activity. Involved in several processes, including positive regulation of cold-induced thermogenesis; sterol homeostasis; and tarsal gland development. Acts upstream of or within several processes, including brown fat cell differentiation; monounsaturated fatty acid biosynthetic process; and white fat cell differentiation. Located in endoplasmic reticulum membrane. Is expressed in several structures, including adipose tissue; central nervous system; cranium; integumental system; and liver. Orthologous to human SCD (stearoyl-CoA desaturase).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M32769 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Flow Cytometry (Fixed), 1:50-200
Positive Control
IHC: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
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IHC analysis of SCD1 using anti-SCD1 antibody (M32769).
SCD1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SCD1 Antibody (M32769) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of SCD1 using anti-SCD1 antibody (M32769).
SCD1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SCD1 Antibody (M32769) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-SCD1 Antibody (Monoclonal, 31S66) (M32769)
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