Product Info Summary
SKU: | A04729-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SCG10/STMN2 Antibody Picoband™
View all Stathmin-2/STMN2 Antibodies
SKU/Catalog Number
A04729-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SCG10/STMN2 Antibody Picoband™ catalog # A04729-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCG10/STMN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04729-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SCG10/STMN2 recombinant protein (Position: S23-N170).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04729-2 is reactive to STMN2 in Human, Mouse, Rat
Applications
A04729-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
21 kDa
Calculated molecular weight
20.828kDa
Background of Stathmin-2/STMN2
Stathmin-2 is a protein that in humans is encoded by the STMN2 gene. This gene encodes a member of the stathmin family of phosphoproteins. Stathmin proteins function in microtubule dynamics and signal transduction. The encoded protein plays a regulatory role in neuronal growth and is also thought to be involved in osteogenesis. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (A04729-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCG10/STMN2 antigen affinity purified polyclonal antibody (Catalog # A04729-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCG10/STMN2 at approximately 21KD. The expected band size for SCG10/STMN2 is at 21KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of Neuro-2a cells using anti-SCG10/STMN2 antibody (A04729-2).
Overlay histogram showing Neuro-2a cells stained with A04729-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (A04729-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of U20S cells using anti-SCG10/STMN2 antibody (A04729-2).
Overlay histogram showing U20S cells stained with A04729-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (A04729-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. IHC analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (A04729-2).
SCG10/STMN2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SCG10/STMN2 Antibody (A04729-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (A04729-2).
SCG10/STMN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SCG10/STMN2 Antibody (A04729-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (A04729-2).
SCG10/STMN2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SCG10/STMN2 Antibody (A04729-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For STMN2 (Source: Uniprot.org, NCBI)
Gene Name
STMN2
Full Name
Stathmin-2
Weight
20.828kDa
Superfamily
stathmin family
Alternative Names
FLJ34868; FLJ50995; neuron-specific growth-associated protein; Protein SCG10; SCG10; SCG10neuronal growth-associated protein (silencer element); SCGN10; SCGN10stathmin-2; Stathmin2; Stathmin-2; stathmin-like 2; STMN2; superior cervical ganglia, neural specific 10; Superior cervical ganglion-10 protein STMN2 SCG10, SCGN10 stathmin 2 stathmin-2|neuron-specific growth-associated protein|neuronal growth-associated protein (silencer element)|stathmin-like 2|superior cervical ganglia, neural specific 10|superior cervical ganglion-10 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STMN2, check out the STMN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STMN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCG10/STMN2 Antibody Picoband™ (A04729-2)
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