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- Table of Contents
AEBP2 antibody (PB9977) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of SiHa cells using anti-AEBP2 antibody (PB9977).
Overlay histogram showing SiHa cells stained with PB9977 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AEBP2 Antibody (PB9977,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with PB9977 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AEBP2 Antibody (PB9977,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-AEBP2 Antibody Picoband®
AEBP2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
AEBP2 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human HL-60 whole cell, human RT4 whole cell, human SiHa whole cell, human CACO-2 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AEBP2 antibody was positively detected by IHC in:
human lung cancer tissue, human mammary cancer tissue, human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AEBP2 antibody was positively detected by ICC/IF in:
U20S cell
AEBP2 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-AEBP2 Antibody Picoband®
View all AEBP2 Antibodies
View all AEBP2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AEBP2 recombinant protein (Position: K424-Q517). Human AEBP2 shares 98.8% amino acid (aa) sequence identity with mouse AEBP2.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
54.5 kDa
Background of AEBP2
Adipocyte Enhancer-Binding Protein is a zinc finger protein that in humans is encoded by the evolutionarily well-conserved gene AEBP2. This gene is mapped to 12p12.3. AEBP2 is a DNA-binding transcriptional repressor. It may regulate the migration and development of the neural crest cells through the PRC2-mediated epigenetic mechanism and is most likely a targeting protein for the mammalian PRC2 complex.
Cite This Product
Anti-AEBP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9977)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Sheep
99%
High Homology
Rat
97%
High Homology
Mouse
85%
Moderate Homology
Zebrafish
65%
Low homology
Drosophila
22%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AEBP2 antibody
Immunohistochemistry (IHC) protocol for AEBP2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AEBP2 antibody
Flow Cytometry (FC) protocol for AEBP2 antibody
Publications
Application:
Species:
PRC2.1 Coordinates Peri-Nucleolar H3K27me3-Enriched Heterochromatin Organization and NPM1 Pentamerization to Maintain Nucleolar Integrity
Published: 2026-06-16
Journal: Advanced Science
Application: WB,IF,CoIP
Impact Factor: 14.10
Species: Human
PMID: 42299771
Review
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Product Promise
We promise Anti-AEBP2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9977
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026