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Product Info Summary
| SKU: | PB9230 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCAK/KIF2C Antibody Picoband®
SKU/Catalog Number
PB9230
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCAK/KIF2C Antibody Picoband® catalog # PB9230. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCAK/KIF2C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9230)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9230 is reactive to KIF2C in Human, Mouse, Rat
Observed Molecular Weight
81 kDa
Calculated molecular weight
81.3 kDa
Background of KIF2C
Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9230 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell
IHC: Mouse Testis Tissue, Rat Testis Tissue, Rat Thymus Tissue
ICC/IF: U20S cell
FCM: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of MCAK using anti-MCAK antibody (PB9230).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAK antigen affinity purified polyclonal antibody (Catalog # PB9230) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCAK at approximately 81 kDa. The expected band size for MCAK is at 81 kDa.
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IHC analysis of KIF2C using anti-KIF2C antibody (PB9230).
KIF2C was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KIF2C Antibody (PB9230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-MCAK antibody, PB9230, IHC(P)
IHC(P): Rat Thymus Tissue
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Anti-MCAK antibody, PB9230, IHC(P)
IHC(P): Rat Testis Tissue
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Anti-MCAK antibody, PB9230, IHC(P)
IHC(P): Mouse Testis Tissue
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IF analysis of MCAK using anti-MCAK antibody (PB9230).
MCAK was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCAK Antibody (PB9230) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of K562 cells using anti-MCAK antibody (PB9230).
Overlay histogram showing K562 cells stained with PB9230 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAK Antibody (PB9230,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-MCAK/KIF2C Antibody Picoband® (PB9230)
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Customer Q&As
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8 Customer Q&As for Anti-MCAK/KIF2C Antibody Picoband®
Question
Does PB9230 anti-MCAK/KIF2C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Taylor
Verified customer
Asked: 2020-01-16
Answer
As indicated on the product datasheet, PB9230 anti-MCAK/KIF2C antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
Is there a BSA free version of anti-MCAK/KIF2C antibody PB9230 available?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MCAK/KIF2C antibody PB9230 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-19
Question
We are currently using anti-MCAK/KIF2C antibody PB9230 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The anti-MCAK/KIF2C antibody (PB9230) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-05
Question
What DAB substrate was used for the images for PB9230?
Verified customer
Asked: 2019-06-11
Answer
For the Anti-MCAK/KIF2C Antibody Picoband™ (PB9230), we used AR1022 to developing the images. https://www.bosterbio.com/dab-chromogenic-kit-brown-ar1022-boster.html
Boster Scientific Support
Answered: 2019-06-11
Question
Will anti-MCAK/KIF2C antibody PB9230 work on pig IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Our lab technicians have not validated anti-MCAK/KIF2C antibody PB9230 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MCAK/KIF2C antibody PB9230 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-MCAK/KIF2C antibody PB9230 work for IHC with testis?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
According to the expression profile of testis, KIF2C is highly expressed in testis. So, it is likely that anti-MCAK/KIF2C antibody PB9230 will work for IHC with testis.
Boster Scientific Support
Answered: 2017-10-05
Question
Is a blocking peptide available for product anti-MCAK/KIF2C antibody (PB9230)?
K. Jones
Verified customer
Asked: 2015-09-09
Answer
We do provide the blocking peptide for product anti-MCAK/KIF2C antibody (PB9230). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-09-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-MCAK/KIF2C antibody PB9230. Let me know if you need anything else.
P. Anderson
Verified customer
Asked: 2014-07-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-09
