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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human LTBR ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human LTBR ELISA Kit PicoKine™ (96 Tests). Quantitate Human LTBR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LTBR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2002)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: Q31-M227
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2002 is reactive to LTBR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK2002 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta receptor (LTBR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3), is a cell surface receptor for lymphotoxin involved in apoptosis and cytokine release. This gene is mapped to 12p13.31. This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
|EK2002-CAP||Anti-Human LTBR Pre-coated 96-well strip microplate||1|
|EK2002-ST||Human LTBR Standard||2 vials, 10 ng/tube|
|EK2002-DA||Human LTBR Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human LTBR PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lymphotoxin beta R/TNFRSF3, Dilution ratio of 1:1, concentration in serum is around 300pg/ml around 60pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LTBR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Lymphotoxin beta R/TNFRSF3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LTBR (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 3
CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III LTBR D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3 lymphotoxin beta receptor tumor necrosis factor receptor superfamily member 3|lymphotoxin B receptor|lymphotoxin beta receptor (TNFR superfamily, member 3)|tumor necrosis factor C receptor|tumor necrosis factor receptor 2-related protein|tumor necrosis factor receptor type III*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LTBR, check out the LTBR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LTBR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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