Anti-SERCA1 ATPase/ATP2A1 Antibody
Rabbit IgG polyclonal antibody for Sarcoplasmic/endoplasmic reticulum calcium ATPase 1(ATP2A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-SERCA1 ATPase/ATP2A1 Antibody
See all ATP2A1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for 1257 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. 1257 information: Molecular Weight: 110252 MW; Subcellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein; Tissue Specificity: Skeletal muscle, fast twitch muscle (type II) fibers.|
|Cite This Product||Anti-SERCA1 ATPase/ATP2A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1719)|
|Specificity||Anti-SERCA1 ATPase/ATP2A1 Antibody (PA1719) reacts with Human, Mouse, Rat ATP2A1, in native form and recombinant. Superfamily members of ATP2A1 are not reactive to PA1719.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SERCA1 ATPase(665-680aa EQREACRRACCFARVE), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-SERCA1 ATPase/ATP2A1 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-SERCA1 ATPase/ATP2A1 Antibody (PA1719).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-SERCA1 ATPase antibody, PA1719, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: PANC Cell Lysate
Lane 3: U87 Cell Lysate
Anti-SERCA1 ATPase antibody, PA1719, IHC(P)
IHC(P): Human Placenta Tissue
Anti-SERCA1 ATPase antibody, PA1719, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Sarcoplasmic/endoplasmic reticulum calcium ATPase 1|
|Tissue Specificity||Skeletal muscle, fast twitch muscle (type II) fibers.|
|Alternative Names||Sarcoplasmic/endoplasmic reticulum calcium ATPase 1;SERCA1;SR Ca(2+)-ATPase 1;220.127.116.11;Calcium pump 1;Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca(2+) ATPase;ATP2A1;|
|Subcellular Localization||Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.|
|Molecular Weight||110252 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||SERCA1(SARCOPLASMIC RETICULUM Ca(2+)-ATPase 1), also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped on 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Zhang et al.(1995)determined that the human ATP2A1 gene is 26 kb long and contains 23 exons, 1 of which can be alternatively spliced.Overexpression of S1T, but not full-length SERCA1, induced ER stress in HeLa cells and amplified ER stress through the PERK(EIF2AK3)-EIF2A -ATF4 -CHOP(DDIT3) pathway. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.