ATP2A1 Antibodies

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1)

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen.

Contributes to calcium sequestration involved in muscle excitation/contraction. .

Gene Information

Human
Gene Name:	ATP2A1
Uniprot:	O14983
Entrez:	487

Family

Belongs to:
cation transport ATPase (P-type) (TC 3.A.3) family

Alternative Names

ATP2A; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform; EC 3.6.3; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1EC 3.6.3.8; SR Ca(2+)-ATPase 1

Molecular Weight

Mass (kDA):
110.252 kDA

Genomic Context

Human
Location:	16p11.2
Sequence:	16; NC_000016.10 (28878488..28904511)

Tissue Specificity

Skeletal muscle, fast twitch muscle (type II) fibers.

Subcellular Localizations

Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

Diseases

• Endometriosis, Site Unspecified
• Myopathy
• Observation Of Neuromuscular Block
• Dystrophy
• Muscle Twitch
• Heart Failure
• Diabetes Mellitus
• Myotonic Dystrophy
• Disorder Of Skeletal Muscle
• Diabetes Mellitus, Non-insulin-dependent
• Malignant Neoplasms
• Keratosis Follicularis

• Muscular Dystrophy
• Isaacs Syndrome
• Hypertrophy
• Obesity
• Cytomegalovirus Infections
• Ischemia
• Nervousness

Pathways

• Transport
• Localization
• Aging
• Muscle Contraction
• Sensitization
• Innervation
• Pathogenesis
• Relaxation Of Vascular Smooth Muscle
• Gene Silencing
• Immune Response
• Swimming Behavior
• Flight
• Mrna Splicing

• Swimming
• Translation
• Exocytosis
• Secretory Pathway
• Cell Proliferation
• Gastrulation
• Insulin Secretion

PTMs

• Oxidation
• Phosphorylation
• Nitration
• Nitrosylation

• Reduction
• Hydroxylation
• Cleavage
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATP2A1

Best Uses Of The ATP2A1 Marker From Boster Bio

ATP2A1 (SERCA Ca (2+),-ATPase) is found in the sarcoplasmic retina of muscle cells. The enzyme catalyzes hydrolysis of ATP as well as translocation of calcium to various locations within the cell. It is involved during the contraction and excitation of muscles. Boster Bio validates antibodies using known positive as well as negative samples to ensure specificity.

ATP2A1 a SERCA Ca (2+).-ATPase

The intracellular calcium transporter ATP2A1 is a protein called ATP2A1. It is located in the sarcoplasmic retina of muscle cells and participates in calcium transport. Brody disease, a condition that can be caused by mutations in the SERCA Ca gene (a member the SERCA family), can occur. ATP2A1 also plays a role in the signaling pathway and the calcium/calcium-mediated disease pathway.

The ATP2A1 protein is an intracellular membrane transporter that uses free energy derived from ATP hydrolysis to transport two Ca ions per ATP. It plays a key role in cell calcium homeostasis and signaling as well as many other physiological processes. In rabbit muscle, extensive research on the ATP2A1 proteins has been conducted. The protein has been shown to be inactivated by several chemicals.

The ATP2A1 proteins has three distinct cytoplasmic regions and ten helical segment. In its functional state, the SERCA transport cycle is characterized by an E1-E2-P scheme, with the high affinity binding of two Ca2+ ions in the cytosol triggering the ATP-dependent hydrolysis of the phosphoenzyme.

SERCA plays a significant role in many diseases. Along with Brody's and Darier's diseases, SERCA mutations are linked to heart failure, cancer, diabetes, and other conditions. SERCA-positive genes are found in all these conditions. Their expression is therefore highly relevant to many human disorders. It has also been identified in myotonic dystrophy.

A sudden rise in ATP concentration triggers SERCA activation. This causes a current transient that is related to charge displacement across ATPase. The SERCA activation triggers is triggered when ATP concentration jumps on SERCA-containing vesicles. And the activation of SERCA results in the production of a SERCA-dependent calcium signal.

PfATP6 is a SERCA-orthogonal of the parasite Plasmodium Falciparum. This parasite is responsible to the majority of malaria-related death worldwide. PfATP6 makes a great target for antimalarial medicines. PfATP6 is more prone DBHQ than rabbitSERCA1a, and its binding affinity with ATP6 significantly higher.

Applications

A bovine ATP2A1 gene mutation is found in exon 8 of the ATP2A1 gene. These variants are genotyped by the ATP2A1 and flanking intronic sequencings. A PCR product containing bovine-ATP2A1 exons 6 and 8 was genotyped. The PCR products could be analyzed using the tools PoPMuSiC for protein stability prediction and NetTurnP and Phyre2 and Phyre3 for transmembranedomain prediction.

In Romagnola cattle, ATP2A1 gene activity has been shown to be associated with decreased SERCA1 activity. By performing selection against the mutant alleles, this mutation can be eliminated from the Romagnola breed. This mutation could be used for research into enterocyte progenitor and stem cell research. These results have important clinical implications in understanding disease progression. The expression of ATP2A1 may be used to identify the neuromuscular disease-causing role of this gene in embryonic stem cell culture.

Primary antibodies

The Boster Bio ATP2A1 marker is a versatile and highly sensitive primary antibody that can be used to identify a broad range of proteins. This antibody allows for more questions to be answered with a single specimen. You can also get contextual data and more reliable answers. Additionally, ATP2A1 is present in all tissues and cells of interest, making it ideal for detecting a variety of diseases and disorders.

In addition to its high affinity, the Boster ATP2A1 primary antibody is well-validated in Western blotting, immunohistochemistry, and ELISA. It can also detect anti-ATP2A1 antibodies within human fibroblasts or pancreatic cancer cells. Scientists can use the Boster ATP2A1 marker as a single or multiparasite antibody to detect the protein in a variety of cell types, including human cells and rats.

Primary antibodies are subsets within the immunoglobulin family that bind to specific antigens. Each antibody is unique in that each antibody has a specific epitope at its surface that determines its affinity or specificity. The two are related. However the more unintended or unwanted antigens they bind, the higher their affinity. The good news is that the Boster Bio APT2A1 marker can be used to measure primary antibodies in blood, serum, or eggs of your subjects.

The primary BMMs were differentiated for four days and stained with the NFATc1 antibody (1:250). Secondary antibody was a goat anti-mouse IgG H&L antibodies. The Boster bio ATP2A1 primary antibody marker provides the best immunoreactive results. Monoclonal antibodies are also available for the bovine NFATc1 antibody.

Gene infographics

If you are a biology student or would like to learn more about the human genome, Gene infographics from Boster Bio may be the perfect resource. These graphics provide basic information regarding each gene and can be used to cover both mouse and human genes. The gene infographics can be accessed in the search bar. You can also search for a specific Gene using the search box. You'll be amazed at the variety of options available. Boster Bio's Gene Infographics cover all genes of the mouse and human.