Anti-SIPA1L1 Antibody

Product Name

Anti-SIPA1L1 Antibody

View all SIPA1L1 Antibodies

SKU/Catalog Number

A06716

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SIPA1L1 Antibody (Catalog # A06716). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SIPA1L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SIPA1L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06716)

Host

Rabbit

Contents

SIPA1L1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SIPA1L1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SIPA1L1. The immunogen is located within amino acids 40 - 90 of SIPA1L1.

Reactive Species

A06716 is reactive to SIPA1L1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

200.0 kDa

Background of SIPA1L1

Signal-induced proliferation associated-like protein 1 (SIPA1L1) is a member of the SIPA1 family of RapGAPs. SIPA1L1 was initially identified as a binding partner and degradation target of the E6 oncoprotein of high-risk papillomaviruses. Recently, it was discovered that Casein kinase I epsilon (CKIe), a Wnt-regulated kinase that regulates Wnt/b-catenin signaling, also can bind to the carboxy-terminus of SIPA1L1. CKIe phosphorylates SIPA1L1, thereby reducing its stability and alleviating its inhibition of Rap1, a protein required for Wnt8/CKIe-mediated gastrulation during embryogenesis, suggesting SIPA1L1 plays important roles in embryo development as well as control of cell proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06716 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SIPA1L1 antibody can be used for detection of SIPA1L1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SIPA1L1

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