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Product Info Summary
| SKU: | M02395-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-SIRT5 Mouse Monoclonal Antibody [Clone ID: OTI2F1]
SKU/Catalog Number
M02395-1
Size
100 µl
Description
Boster Bio SIRT5 mouse monoclonal antibody, clone OTI2F1 (formerly 2F1). Catalog# M02395-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SIRT5 Mouse Monoclonal Antibody [Clone ID: OTI2F1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02395-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2F1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293 cell.
Reactive Species
M02395-1 is reactive to SIRT5 in Human, Monkey
Observed Molecular Weight
34 kDa
Calculated molecular weight
33.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02395-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC: 1:150
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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Western blot analysis of SIRT5 using anti-SIRT5 antibody (M02395-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SIRT5 antigen affinity purified monoclonal antibody (Catalog # M02395-1) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SIRT5 at approximately 34 kDa. The expected band size for SIRT5 is at 34 kDa.
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Anti-SIRT5 mouse monoclonal antibody (M02395-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.
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HEK293T cells transfected with either SIRT5 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody (M02395-1)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Specific Publications For Anti-SIRT5 Mouse Monoclonal Antibody [Clone ID: OTI2F1] (M02395-1)
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