Rabbit IgG polyclonal antibody for Solute carrier family 22 member 6(SLC22A6) detection. Tested with WB, IHC-P in Human;Rat.
Anti-SLC22A6 Antibody Info At A Glance
|Product Name||Anti-SLC22A6 Antibody
See all SLC22A6 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for OAT1/SLC22A6 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. OAT1/SLC22A6 information: Molecular Weight: 61816 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein ; Tissue Specificity: Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.|
|Cite This Product||Anti-SLC22A6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1683)|
|Similar Products From
|Anti-SLC22A6 Antibody may replace the following items: sc 161978.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SLC22A6(534-550aa QKYMVPLQASAQEKNGL), different from the related rat sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For SLC22A6 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||9356|
|Species Of This Entry||Human|
|Protein Name||Solute carrier family 22 member 6|
|Superfamily||major facilitator (TC 2.A.1) superfamily|
|Alternative Names||SLC22A6|hOAT1; hPAHT; hROAT1; MGC45260; OAT1; OAT1FLJ55736; Organic anion transporter 1; PAH transporter; PAHT; PAHTHOAT1; para-aminohippurate transporter; Renal organic anion transporter 1; ROAT1; SLC22A6; solute carrier family 22 (organic anion transporter), member 6; solute carrier family 22 member 6|
|Post Tranlational Modifications||Carboxylation; Phosphorylation; Reduction; Glycosylation|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Gene Location||11q12.3, on Chromasome 11, gene sequence: NC_000011.10 (62976597..62984967, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SLC22A6 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p- aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido- 3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro- phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2- furanpropionate (CMPF), cidofovir, adefovir, 9-(2- phosphonylmethoxyethyl) guanine (PMEG), 9-(2- phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p- chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.|
|Tissue Specificity||Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.|
|Related Diseases||Kidney Failure; Acute Kidney Injury; Kidney Diseases; Injury To Kidney; Kidney Failure, Acute; Hyperuricemia; Hiv Infections; Reperfusion Injury; Malignant Neoplasm Of Breast; Biliary Obstruction; Malignant Neoplasms; Headache; Ischemia; Cholestasis; Inflammation; Ureteral Obstruction; Uremia; Obstructive Nephropathy; Dysequilibrium Syndrome|
|Related Pathways||Transport; Excretion; Secretion; Glomerular Filtration; Organic Anion Transport; Anion Transport; Drug Transport; Conjugation; Localization; Renal Tubular Secretion; Ion Transport; Kidney Development; Methotrexate Transport; Cytokine Secretion; Sulfate Transport; Intestinal Absorption; Transepithelial Transport; Hormone Transport; Thyroid Hormone Transport; Reverse Transcription|
|Background||SLC22A6(Solute carrier family 22(organic anion transporter), member 6), also called OAT1 or PAHT, is a protein that in humans is encoded by the SLC22A6 gene, which is also a member of the organic anion transporter(OAT) family of proteins. OAT1 is a transmembrane protein that is expressed in the brain, the placenta, the eyes, smooth muscles, and the basolateral membrane of proximal tubular cells of the kidneys. The SLC22A6 gene is mapped on 11q12.3. It plays a central role in renal organic anion transport. Along with OAT3, OAT1 mediates the uptake of a wide range of relatively small and hydrophilic organic anions from plasma into the cytoplasm of the proximal tubular cells of the kidneys. The SLC22A6 gene contains 10 exons and spans 8.2 kb. OAT1 functions as organic anion exchanger. When the uptake of one molecule of an organic anion is transported into a cell by an OAT1 exchanger, one molecule of an endogenous dicarboxylic acid(such as glutarate, ketoglutarate, etc) is simultaneously transported out of the cell. PAH uptake in Xenopus oocytes injected with OAT1 mRNA was demonstrated by Race et al.|
|Scientific References||PMID: 9762842 by Reid G., et al. Cloning of a human renal p-aminohippurate transporter, hROAT1.|
PMID: 9887087 by Hosoyamada M., et al. Molecular cloning and functional expression of a multispecific organic anion transporter from human kidney.
Our Boster Quality Guarantee for Anti-SLC22A6 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Rat Kidney Tissue
Lane 1: HT1080 Cell Lysate
Lane 2: HELA Cell Lysate
SLC22A6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- SLC22A6 Antibody (PA1683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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