Anti-Replication factor C subunit 1 RFC1 Antibody
Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
Anti-Replication factor C subunit 1 RFC1 Antibody Info At A Glance
|Product Name||Anti-Replication factor C subunit 1 RFC1 Antibody
See all RFC1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for RFC1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. RFC1 information: Molecular Weight: 128255 MW; Subcellular Localization: Nucleus; Tissue Specificity: Wide tissue distribution. Undetectable in placental tissue.|
|Cite This Product||Anti-Replication factor C subunit 1 RFC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1716)|
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|Anti-Replication factor C subunit 1 RFC1 Antibody may replace the following items: sc 20993|sc 21878|sc 271656|sc 11909|sc 11908.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1(46-64aa NSSRKEDDFKQKQPSKKKR).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For RFC1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5981|
|Species Of This Entry||Human|
|Protein Name||Replication factor C subunit 1|
|Superfamily||activator 1 large subunit family|
|Alternative Names||RFC1|A1 140 kDa subunit; A1; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; MGC51786; MHC binding factor, beta; MHCBFB; PO-GA; RECC1; replication factor C (activator 1) 1 (145kD); replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C large subunit; replication factor C subunit 1; replication factor C1; RF-C 140 kDa subunit; RFC; RFC1; RFC140; RFC140DNA-binding protein PO-GA|
|Post Tranlational Modifications||Methylation; Phosphorylation; Reduction; Ubiquitination; Demethylation; Cleavage; Deacetylation; Acetylation|
|Gene Location||4p14, on Chromasome 4, gene sequence: NC_000004.12 (39287456..39366381, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For RFC1 (Source: Uniprot.org, NCBI)
|Protein Function||The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.|
|Tissue Specificity||Wide tissue distribution. Undetectable in placental tissue.|
|Related Diseases||5,10-methylenetetrahydrofolate Reductase Deficiency; Malignant Neoplasms; Leukemia; Acute Lymphocytic Leukemia; Neoplasms; Malignant Neoplasm Of Breast; L1 Acute Lymphoblastic Leukemia; Leukemia L1210; Folic Acid Deficiency; Congenital Abnormality; Mammary Neoplasms; Carcinoma; Cleft Palate; Cleft Lip; Down Syndrome; Hyperhomocysteinemia; Cardiovascular Diseases; Lymphoma; Dysequilibrium Syndrome; Lung Neoplasms|
|Related Pathways||Transport; Dna Replication; Dna Repair; Cell Cycle; Methylation; Dna Damage Checkpoint; Dna Methylation; S Phase; Localization; Drug Resistance; Cell Proliferation; Mismatch Repair; Sister Chromatid Cohesion; Methotrexate Transport; Cell Cycle Arrest; Cell Growth; Cell Division; Drug Transport; Translesion Synthesis; Mitotic Recombination|
|Background||RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.|
|Scientific References||PMID: 8248204 by Bunz F., et al. cDNAs encoding the large subunit of human replication factor C.|
PMID: 8512577 by Lu Y., et al. Cloning and expression of a novel human DNA binding protein, PO-GA.
Our Boster Quality Guarantee for Anti-Replication factor C subunit 1 RFC1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA Cell Lysate,
Lane 2: SMMC Cell Lysate,
Lane 3: JURKAT Cell Lysate,
Lane 4: CCRF-CEM Cell Lysate,
Lane 5: 293T Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- RFC1 antigen affinity purified polyclonal antibody (Catalog # PA1716) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150KD. The expected band size for RFC1 is at 128KD.
RFC1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RFC1 Antibody (PA1716) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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