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Product Info Summary
| SKU: | M01615-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-SLUG (SNAI2) Mouse Monoclonal Antibody [Clone ID: OTI1A6]
SKU/Catalog Number
M01615-1
Size
100 µl
Description
Boster Bio SNAI2 (SLUG) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6). Catalog# M01615-1. Tested in FC, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SLUG (SNAI2) Mouse Monoclonal Antibody [Clone ID: OTI1A6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01615-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1A6
Isotype
IgG1
Immunogen
Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
Reactive Species
M01615-1 is reactive to SNAI2 in Human, Mouse, Rat
Calculated molecular weight
30.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01615-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000
IHC 1:150
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Figure from citation: Immunohistochemical analysis of Slug (also known as SNAI2) on serial sections of cartilage from normal and OA cartilage by using anti-SNAI2 antibody. Bar=100 um
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either SNAI2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody (M01615-1)
Specific Publications For Anti-SLUG (SNAI2) Mouse Monoclonal Antibody [Clone ID: OTI1A6] (M01615-1)
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