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Product Info Summary
| SKU: | A00285 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-PAX3 Antibody (N-term)
SKU/Catalog Number
A00285
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-PAX3 Antibody (N-term) (Catalog # A00285). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PAX3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00285)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PAX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the N-terminal region of human PAX3.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00285 is reactive to PAX3 in Human
Calculated molecular weight
53.0 kDa
Background of PAX3
PAX3 probable is a transcription factor associated with development of alveolar rhabdomyosarcoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00285 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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PAX3 Antibody (N-term) western blot analysis in A431 cell line lysates (35ug/lane). This demonstrates the PAX3 antibody detected the PAX3 protein (arrow).
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Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-PAX3 Antibody (N-term) (A00285)
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