Click image to see more details
Product Info Summary
| SKU: | PA1315 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SNAP25 Antibody Picoband®
SKU/Catalog Number
PA1315
BA1400 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNAP25 Antibody catalog # PA1315. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNAP25 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1315)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1315 is reactive to SNAP25 in Human, Mouse, Rat
Observed Molecular Weight
40-50 kDa, 80-140 kDa
Calculated molecular weight
23.3 kDa
Background of SNAP25
Synaptosome-associated protein of 25,000 daltons also known as SNAP-25 is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. Nagy et al. (2004) identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1315 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Positive Control
WB: Rat Brain Tissue
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-SNAP25 antibody, PA1315, Western blotting
WB: Rat Brain Tissue Lysate
Click image to see more details
Anti-SNAP25 antibody, PA1315, IHC(P)
IHC(P): Rat Brain Tissue
Specific Publications For Anti-SNAP25 Antibody Picoband® (PA1315)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SNAP25 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-SNAP25 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-SNAP25 Antibody Picoband®
Question
Would anti-SNAP25 antibody PA1315 work for IHC with eye?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
According to the expression profile of eye, SNAP25 is highly expressed in eye. So, it is likely that anti-SNAP25 antibody PA1315 will work for IHC with eye.
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-SNAP25 antibody for IHC for mouse eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse eye identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
It shows on the product datasheet, PA1315 anti-SNAP25 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-SNAP25 antibody PA1315. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-26
Question
Can you help my question with product PA1315, anti-SNAP25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Edwards
Verified customer
Asked: 2019-11-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1315 anti-SNAP25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-15
Question
Our team were satisfied with the WB result of your anti-SNAP25 antibody. However we have seen positive staining in frontal cortex cytoplasm using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
Based on literature, frontal cortex does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2019-10-14
Question
We are currently using anti-SNAP25 antibody PA1315 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-SNAP25 antibody (PA1315) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
Would anti-SNAP25 antibody PA1315 work on dog IHC with brain?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Our lab technicians have not tested anti-SNAP25 antibody PA1315 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SNAP25 antibody PA1315 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-14
Question
Will PA1315 anti-SNAP25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
It shows on the product datasheet, PA1315 anti-SNAP25 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-18
Question
We want to test anti-SNAP25 antibody PA1315 on mouse eye for research purposes, then I may be interested in using anti-SNAP25 antibody PA1315 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Krishna
Verified customer
Asked: 2018-10-05
Answer
The products we sell, including anti-SNAP25 antibody PA1315, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-05
Question
Our lab want to know about using your anti-SNAP25 antibody for glutamate secretion studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
Thanks for your inquiry. This PA1315 anti-SNAP25 antibody is tested on rat brain tissue, tissue lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-09-03
Question
Do you have a BSA free version of anti-SNAP25 antibody PA1315 available?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP25 antibody PA1315 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-30
Question
We bought anti-SNAP25 antibody for IHC on frontal cortex in a previous project. I am using human, and I plan to use the antibody for WB next. I am interested in examining frontal cortex as well as skeletal muscle in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
I took a look at the website and datasheets of our anti-SNAP25 antibody and it appears that PA1315 has been tested on human in both IHC and WB. Thus PA1315 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-24
Question
Is a blocking peptide available for product anti-SNAP25 antibody (PA1315)?
J. Evans
Verified customer
Asked: 2018-02-01
Answer
We do provide the blocking peptide for product anti-SNAP25 antibody (PA1315). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-01
Question
I see that the anti-SNAP25 antibody PA1315 works with IHC, what is the protocol used to produce the result images on the product page?
H. Walker
Verified customer
Asked: 2017-12-05
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-12-05
Question
Is this PA1315 anti-SNAP25 antibody reactive to the isotypes of SNAP25?
K. Dhar
Verified customer
Asked: 2017-04-25
Answer
The immunogen of PA1315 anti-SNAP25 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25(186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-25
Question
Here is the WB image, lot number and protocol we used for eye using anti-SNAP25 antibody PA1315. Please let me know if you require anything else.
T. Anderson
Verified customer
Asked: 2016-01-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-28
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-SNAP25 antibody supposed to stain brain positively?
F. Lewis
Verified customer
Asked: 2015-11-04
Answer
From literature brain does express SNAP25. From Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2015-11-04
