Anti-SNAP25 Antibody

Boster Bio Anti-SNAP25 Antibody catalog # PA1315. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1315
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-SNAP25 Antibody

See all SNAP25 products

SKU/Catalog Number

PA1315

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SNAP25 Antibody catalog # PA1315. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SNAP25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1315)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25 (186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1315 is reactive to SNAP25 in Human, Mouse, Rat

Applications

PA1315 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For SNAP25 (Source: Uniprot.Org, NCBI)

Uniprot ID

P60880

Gene ID

6616

Gene Name

SNAP25

Full Name

Synaptosomal-associated protein 25

Weight

23.315kDa

Superfamily

SNAP-25 family

Alternative Names

bA416N4.2; dJ1068F16.2; FLJ23079; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP25; SNAP-25SNAP; SUP; Super protein; Synaptosomal-associated 25 kDa protein; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa SNAP25 CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2 synaptosome associated protein 25 synaptosomal-associated protein 25|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein, 25kDa|synaptosome associated protein 25kDa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SNAP25, check out the SNAP25 Infographic

SNAP25 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SNAP25: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1315 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
PA1315 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:250

Zhong ZQ, Xiang Y, Hu X, Wang YC, Zeng X, Wang XM, Xia QJ, Wang TH, Zhang X. Neural Regen Res. 2017 Jun;12(6):969-976. doi: 10.4103/1673-5374.208592. Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomo...

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17 Customer Q&As for Anti-SNAP25 Antibody

Question

Would anti-SNAP25 antibody PA1315 work for IHC with eye?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

According to the expression profile of eye, SNAP25 is highly expressed in eye. So, it is likely that anti-SNAP25 antibody PA1315 will work for IHC with eye.

Boster Scientific Support

Answered: 2020-02-27

Question

I was wanting to use your anti-SNAP25 antibody for IHC for mouse eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse eye identification?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

It shows on the product datasheet, PA1315 anti-SNAP25 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse eye in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-11

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-SNAP25 antibody PA1315. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-26

Question

Can you help my question with product PA1315, anti-SNAP25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Edwards

Verified customer

Asked: 2019-11-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1315 anti-SNAP25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-15

Question

Our team were satisfied with the WB result of your anti-SNAP25 antibody. However we have seen positive staining in frontal cortex cytoplasm using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

Based on literature, frontal cortex does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387

Boster Scientific Support

Answered: 2019-10-14

Question

We are currently using anti-SNAP25 antibody PA1315 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

The anti-SNAP25 antibody (PA1315) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-10

Question

Would anti-SNAP25 antibody PA1315 work on dog IHC with brain?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

Our lab technicians have not tested anti-SNAP25 antibody PA1315 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SNAP25 antibody PA1315 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-14

Question

Will PA1315 anti-SNAP25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-18

Answer

It shows on the product datasheet, PA1315 anti-SNAP25 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-18

Question

We want to test anti-SNAP25 antibody PA1315 on mouse eye for research purposes, then I may be interested in using anti-SNAP25 antibody PA1315 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Krishna

Verified customer

Asked: 2018-10-05

Answer

The products we sell, including anti-SNAP25 antibody PA1315, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-10-05

Question

Our lab want to know about using your anti-SNAP25 antibody for glutamate secretion studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-09-03

Answer

Thanks for your inquiry. This PA1315 anti-SNAP25 antibody is tested on rat brain tissue, tissue lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-09-03

Question

Do you have a BSA free version of anti-SNAP25 antibody PA1315 available?

Verified Customer

Verified customer

Asked: 2018-07-30

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP25 antibody PA1315 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-30

Question

We bought anti-SNAP25 antibody for IHC on frontal cortex in a previous project. I am using human, and I plan to use the antibody for WB next. I am interested in examining frontal cortex as well as skeletal muscle in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-07-24

Answer

I took a look at the website and datasheets of our anti-SNAP25 antibody and it appears that PA1315 has been tested on human in both IHC and WB. Thus PA1315 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-07-24

Question

Is a blocking peptide available for product anti-SNAP25 antibody (PA1315)?

J. Evans

Verified customer

Asked: 2018-02-01

Answer

We do provide the blocking peptide for product anti-SNAP25 antibody (PA1315). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-01

Question

I see that the anti-SNAP25 antibody PA1315 works with IHC, what is the protocol used to produce the result images on the product page?

H. Walker

Verified customer

Asked: 2017-12-05

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-05

Question

Is this PA1315 anti-SNAP25 antibody reactive to the isotypes of SNAP25?

K. Dhar

Verified customer

Asked: 2017-04-25

Answer

The immunogen of PA1315 anti-SNAP25 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25(186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-04-25

Question

Here is the WB image, lot number and protocol we used for eye using anti-SNAP25 antibody PA1315. Please let me know if you require anything else.

T. Anderson

Verified customer

Asked: 2016-01-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-01-28

Question

We have observed staining in human brain. Do you have any suggestions? Is anti-SNAP25 antibody supposed to stain brain positively?

F. Lewis

Verified customer

Asked: 2015-11-04

Answer

From literature brain does express SNAP25. From Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387

Boster Scientific Support

Answered: 2015-11-04

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