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Product Info Summary
| SKU: | A03348-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Neurotrophin 3/NTF3 Antibody Picoband®
SKU/Catalog Number
A03348-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neurotrophin 3/NTF3 Antibody Picoband® catalog # A03348-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurotrophin 3/NTF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03348-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Neurotrophin 3/NTF3, which shares 89.3% and 95.3% amino acid (aa) sequence identity with mouse and rat Neurotrophin 3/NTF3, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03348-2 is reactive to NTF3 in Human, Mouse, Rat
Observed Molecular Weight
34 kDa
Calculated molecular weight
29.4 kDa
Background of NTF3
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03348-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue, human U87 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of Neurotrophin 3/NTF3 using anti-Neurotrophin 3/NTF3 antibody (A03348-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotrophin 3/NTF3 antigen affinity purified polyclonal antibody (Catalog # A03348-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotrophin 3/NTF3 at approximately 34KD. The expected band size for Neurotrophin 3/NTF3 is at 34KD.
Specific Publications For Anti-Neurotrophin 3/NTF3 Antibody Picoband® (A03348-2)
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