|Validated Species:||Human, Mouse|
Data & Images
|Product Name||Anti-SOCS3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human;Mouse.|
|Cite This Product||Anti-SOCS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1707)|
|Replacement Item||This antibody may replace the following items: sc-51699|sc-7009|sc-7010|sc-73045|sc-9023 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Suppressor of cytokine signaling 3(SOCS-3)|
|Molecular Weight||24770 MW|
|Protein Function||SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity). .|
|Tissue Specificity||Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.|
|Sequence Similarities||Contains 1 SH2 domain.|
|Alternative Names||Suppressor of cytokine signaling 3;SOCS-3;Cytokine-inducible SH2 protein 3;CIS-3;STAT-induced STAT inhibitor 3;SSI-3;SOCS3;CIS3, SSI3;|
|Research Areas|||immunology|innate immunity|cytokines|il6 related| signal transduction|adapters|cytoplasmic| epigenetics and nuclear signaling|nuclear signaling pathways|stats| stem cells|hematopoietic progenitors|intracellular molecules| cancer|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease|obesity||
Background for Suppressor of cytokine signaling 3(SOCS-3)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SOCS3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SOCS3 Antibody Images
Click the images to enlarge.
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
IHC(P): Human Tonsil Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,