Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human;Mouse. Cited in 2 publication(s).
Anti-SOCS3 Antibody Info At A Glance
|Product Name||Anti-SOCS3 Antibody
See all SOCS-3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for SOCS-3/SOCS3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. SOCS-3/SOCS3 information: Molecular Weight: 24770 MW; Tissue Specificity: Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.|
|Cite This Product||Anti-SOCS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1707)|
|Similar Products From
|Anti-SOCS3 Antibody may replace the following items: sc 51699|sc 7009|sc 7010|sc 73045|sc 9023.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For SOCS3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||9021|
|Species Of This Entry||Human|
|Protein Name||Suppressor of cytokine signaling 3|
|Alternative Names||SOCS-3|ATOD4; CIS3; CIS3CIS-3; Cish3; cytokine-induced SH2 protein 3; Cytokine-inducible SH2 protein 3; SOCS3; SOCS-3; SOCS-3STAT-induced STAT inhibitor 3; SSI-3; SSI-3ATOD4; SSI3MGC71791; suppressor of cytokine signaling 3|
|Post Tranlational Modifications||Phosphorylation; Methylation; Ubiquitination; Oxidation; Demethylation; Dephosphorylation; Acetylation; Reduction; Cleavage; Glycosylation; Isgylation; Farnesylation; Deacetylation; Geranylgeranylation|
|Gene Location||17q25.3, on Chromasome 17, gene sequence: NC_000017.11 (78356777..78360079, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SOCS3 (Source: Uniprot.org, NCBI)
|Protein Function||SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity).|
|Tissue Specificity||Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.|
|Interacting Proteins||GAB1; KIAA1958; KIT; MAP1S; SNAI1; YES1|
|Research Areas||Signal Transduction|
|Related Diseases||Inflammation; Janiceps; Obesity; Malignant Neoplasms; Insulin Resistance; Neoplasms; Leukemia; Diabetes Mellitus; Hepatitis; Inflammatory Response; Carcinoma; Diabetes Mellitus, Non-insulin-dependent; Insulin Sensitivity; Autoimmune Reaction; Liver Carcinoma; Hepatitis C; Arthritis; Autoimmune Diseases; Tissue Adhesions; Infective Disorder|
|Related Pathways||Pathogenesis; Secretion; Immune Response; Cell Proliferation; Inflammatory Response; Methylation; Cytokine Production; Regeneration; Cell Differentiation; Cell Growth; Lactation; Energy Homeostasis; Cell Cycle; Cell Death; Liver Regeneration; Localization; Cell Activation; Glucose Homeostasis; Transport; Hypersensitivity|
|Background||SOCS3(Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon(IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.|
|Scientific References||PMID: 9266833 by Minamoto S., et al. Cloning and functional analysis of new members of STAT induced STAT inhibitor (SSI) family: SSI-2 and SSI-3.|
PMID: 9344848 by Masuhara M., et al. Cloning and characterization of novel CIS family genes.
Our Boster Quality Guarantee for Anti-SOCS3 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
SOCS3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOCS3 Antibody (PA1707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (Catalog # PA1707) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 30KD. The expected band size for SOCS3 is at 25KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Wan J, Che Y, Kang N, Wu W. Mol Med Rep. 2015 Jul;12(1):83-92. Doi: 10.3892/Mmr.2015.3368. Epub 2015 Feb 17. Socs3 Blocks Hif-1?? Expression To Inhibit Proliferation And Angiogenesis Of Human Small Cell Lung Cancer By Downregulating Activation Of ...||PubMed ID 25695729|
|Yang M, Tian M, Zhang X, Xu J, Yang B, Yu J, Li F, Li Y, Li S, Li X. Oncotarget. 2017 Jun 16;8(57):96958-96969. doi: 10.18632/oncotarget.18555. eCollection 2017 Nov 14. Role of the JAK2/STAT3 signaling pathway in the pathogenesis of type 2 diabete...||PubMed ID 29228585|
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Note: sample size takes longer to process, average 5-7 days.
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