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- Table of Contents
OCLN antibody (A01246-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of U20S cells using anti-Occludin/OCLN antibody (A01246-2).
Overlay histogram showing U20S cells stained with A01246-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Occludin/OCLN Antibody (A01246-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U20S cells stained with A01246-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Occludin/OCLN Antibody (A01246-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Occludin/OCLN Antibody Picoband®
OCLN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
OCLN antibody was positively detected by WB in:
human HepG2 whole cell, human HEK293 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
OCLN antibody was positively detected by IHC in:
human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
OCLN antibody was positively detected by ICC/IF in:
A431 cell
OCLN antibody was positively detected by IP in:
HepG2 cell
OCLN antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Occludin/OCLN Antibody Picoband®
View all OCLN Antibodies
View all OCLN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Occludin/OCLN recombinant protein (Position: H54-K270).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
59.1 kDa
Background of OCLN
Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.
Cite This Product
Anti-Occludin/OCLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01246-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Sheep
92%
High Homology
Mouse
91%
High Homology
Rabbit
91%
High Homology
Cow
91%
High Homology
Rat
89%
Moderate Homology
Dog
89%
Moderate Homology
Zebrafish
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for OCLN antibody
Immunohistochemistry (IHC) protocol for OCLN antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for OCLN antibody
Immunoprecipitation (IP) protocol for OCLN antibody
Flow Cytometry (FC) protocol for OCLN antibody
Publications
Application:
Species:
Astragaloside lV-Loaded Mesoporous Silica Nanoparticles Attenuated Cerebral Ischemic Injury via Iron Overload and Neuroinflammation
Published: 2026-06-15
Journal: ACS Applied Nano Materials
Application: IHC
Impact Factor: 5.80
Species: Mouse
Rosmarinic acid Liposomes Suppress Ferroptosis in Ischemic Brain via Inhibition of TfR1 in BMECs
Published: 2024-06-28
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.70
Species: Mouse
PMID: 38968791
Probiotics-loaded microcapsules from gas-assisted microfluidics for inflammatory bowel disease treatment
Published: 2023-05-22
Journal: MACROMOLECULAR RESEARCH
Application: IF
Impact Factor: 2.40
Species: Mouse
Probiotics-loaded carrageenan microspheres for inflammatory bowel disease treatment
Published: 2023-02-17
Journal: Materials Research Express
Application: IHC
Impact Factor: 2.30
Species: Mouse
Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia
Published: 2022-05-19
Journal: Frontiers in Immunology
Application: IF
Impact Factor: 8.79
Species: Mouse
PMID: 35664001
Intranasal administration of human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke
Published: 2015-08-13
Journal: BRAIN RESEARCH
Application: IHC
Impact Factor: 2.84
Species: Rat
PMID: 26279113
Age-related changes in small intestinal mucosa epithelium architecture and epithelial tight junction in rat models
Published: 2013-11-17
Journal: AGING CLINICAL AND EXPERIMENTAL RESEARCH
Application: IHC
Impact Factor: 1.01
Species: Rat
PMID: 24243034
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Product Promise
We promise Anti-Occludin/OCLN Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01246-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026