Anti-SOX10 Rabbit Monoclonal Antibody

SOX10 antibody

Boster Bio Anti-SOX10 Rabbit Monoclonal Antibody catalog # M00758-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00758-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-SOX10 Rabbit Monoclonal Antibody

View all SOX10 Antibodies

SKU/Catalog Number

M00758-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-SOX10 Rabbit Monoclonal Antibody catalog # M00758-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SOX10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00758-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GIB-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SOX10

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00758-1 is reactive to SOX10 in Human, Mouse, Rat

Applications

M00758-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

49.911kDa

Background of SOX10

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For SOX10 (Source: Uniprot.org, NCBI)

Gene Name

SOX10

Full Name

Transcription factor SOX-10

Weight

49.911kDa

Alternative Names

DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of SOX10 DOM, PCWH, WS2E, WS4, WS4C SRY-box transcription factor 10 transcription factor SOX-10|SRY (sex determining region Y)-box 10|SRY-box 10|SRY-related HMG-box gene 10|dominant megacolon, mouse, human homolog of

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SOX10, check out the SOX10 Infographic

SOX10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SOX10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M00758-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-SOX10 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-SOX10 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-SOX10 Rabbit Monoclonal Antibody

Question

We ordered your anti-SOX10 Rabbit Monoclonal antibody for WB on placenta skin in the past. I am using human, and We are going to use the antibody for IF next. I was wanting to use examining placenta skin as well as small intestine in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

I took a look at the website and datasheets of our anti-SOX10 Rabbit Monoclonal antibody and it appears that M00758-1 has been validated on human in both WB and IF. Thus M00758-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-29

Question

Can you help my question with product M00758-1, anti-SOX10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

O. Miller

Verified customer

Asked: 2020-03-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00758-1 anti-SOX10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-03

Question

Is a blocking peptide available for product anti-SOX10 Rabbit Monoclonal antibody (M00758-1)?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

We do provide the blocking peptide for product anti-SOX10 Rabbit Monoclonal antibody (M00758-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-13

Question

We have observed staining in rat inferior olivary complex. Are there any suggestions? Is anti-SOX10 Rabbit Monoclonal antibody supposed to stain inferior olivary complex positively?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

From what I have seen in literature inferior olivary complex does express SOX10. From what I have seen in Uniprot.org, SOX10 is expressed in inferior olivary complex, small intestine, placenta skin, among other tissues. Regarding which tissues have SOX10 expression, here are a few articles citing expression in various tissues:
Placenta, and Skin, Pubmed ID: 15489334
Small intestine, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-08

Question

Will anti-SOX10 Rabbit Monoclonal antibody M00758-1 work for IF with small intestine?

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

According to the expression profile of small intestine, SOX10 is highly expressed in small intestine. So, it is likely that anti-SOX10 Rabbit Monoclonal antibody M00758-1 will work for IF with small intestine.

Boster Scientific Support

Answered: 2019-12-26

Question

Does M00758-1 anti-SOX10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

As indicated on the product datasheet, M00758-1 anti-SOX10 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-20

Question

We are currently using anti-SOX10 Rabbit Monoclonal antibody M00758-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The anti-SOX10 Rabbit Monoclonal antibody (M00758-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-04

Question

See attached the WB image, lot number and protocol we used for small intestine using anti-SOX10 Rabbit Monoclonal antibody M00758-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-10

Question

I see that the anti-SOX10 Rabbit Monoclonal antibody M00758-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-04

Question

Is this M00758-1 anti-SOX10 Rabbit Monoclonal antibody reactive to the isotypes of SOX10?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

The immunogen of M00758-1 anti-SOX10 Rabbit Monoclonal antibody is A synthesized peptide derived from human SOX10 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-28

Question

Do you have a BSA free version of anti-SOX10 Rabbit Monoclonal antibody M00758-1 available?

Verified Customer

Verified customer

Asked: 2019-03-22

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SOX10 Rabbit Monoclonal antibody M00758-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-22

Question

Our lab were content with the WB result of your anti-SOX10 Rabbit Monoclonal antibody. However we have been able to see positive staining in inferior olivary complex cytoplasm. using this antibody. Is that expected? Could you tell me where is SOX10 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-01

Answer

Based on literature, inferior olivary complex does express SOX10. Generally SOX10 expresses in cytoplasm. Regarding which tissues have SOX10 expression, here are a few articles citing expression in various tissues:
Placenta, and Skin, Pubmed ID: 15489334
Small intestine, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-06-01

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine using anti-SOX10 Rabbit Monoclonal antibody M00758-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-18

Question

We want to test anti-SOX10 Rabbit Monoclonal antibody M00758-1 on human small intestine for research purposes, then I may be interested in using anti-SOX10 Rabbit Monoclonal antibody M00758-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-13

Answer

The products we sell, including anti-SOX10 Rabbit Monoclonal antibody M00758-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-13

Question

I was wanting to use your anti-SOX10 Rabbit Monoclonal antibody for IF for human small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine identification?

K. Wu

Verified customer

Asked: 2014-10-07

Answer

As indicated on the product datasheet, M00758-1 anti-SOX10 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-10-07

Order DetailsPrice
M00758-1

100μL

$370
M00758-1-30ul

30μL sample

$99
M00758-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00758-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.