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Anti-EDNRB Antibody Picoband™ (monoclonal, 15C3)
Boster Bio Anti-EDNRB Antibody Picoband™ (monoclonal, 15C3) catalog # M01041-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDNRB Antibody Picoband™ (monoclonal, 15C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01041-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human EDNRB (406-439aa QSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKY), different from the related mouse and rat sequences by one amino acid.
No cross reactivity with other proteins.
M01041-1 is reactive to EDNRB in Human
M01041-1 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EDNRB using anti-EDNRB antibody (M01041-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EDNRB antigen affinity purified monoclonal antibody (Catalog # M01041-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EDNRB at approximately 45KD. The expected band size for EDNRB is at 45KD.
Gene/Protein Information For EDNRB (Source: Uniprot.Org, NCBI)
Endothelin receptor type B
G-protein coupled receptor 1 family
ABCDS; EDNRB; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor type B; ETB; ET-B; ETBR; ET-BR; ETRB; HSCR; HSCR2; WS4A EDNRB ABCDS, ET-B, ET-BR, ETB, ETB1, ETBR, ETRB, HSCR, HSCR2, WS4A endothelin receptor type B endothelin receptor type B|Hirschsprung disease 2|endothelin receptor non-selective type|endothelin receptor subtype B1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EDNRB, check out the EDNRB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EDNRB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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