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Product Info Summary
| SKU: | A03441 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-SP-B SFTPB Antibody
SKU/Catalog Number
A03441
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SP-B SFTPB Antibody catalog # A03441. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SP-B SFTPB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03441)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SP-B. AA range:243-292
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03441 is reactive to SFTPB in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
42.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03441 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using SP-B Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysate from A549 cells, using SP-B antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-SP-B SFTPB Antibody (A03441)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SP-B SFTPB Antibody
Question
I see that the anti-SP-B antibody A03441 works with WB, what is the protocol used to produce the result images on the product page?
H. Jones
Verified customer
Asked: 2020-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-SP-B antibody A03441. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
Do you have a BSA free version of anti-SP-B antibody A03441 available?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SP-B antibody A03441 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-06
Question
I was wanting to use your anti-SP-B antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
It shows on the product datasheet, A03441 anti-SP-B antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-08
Question
Does anti-SP-B antibody A03441 work for WB with lung?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
According to the expression profile of lung, SFTPB is highly expressed in lung. So, it is likely that anti-SP-B antibody A03441 will work for WB with lung.
Boster Scientific Support
Answered: 2018-04-30
