Anti-Macrophage metalloelastase MMP12 Antibody

Boster Bio Anti-Macrophage metalloelastase MMP12 Antibody catalog # PA2146. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2146
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Macrophage metalloelastase MMP12 Antibody

See all MMP-12 products

SKU/Catalog Number

PA2146

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Macrophage metalloelastase MMP12 Antibody catalog # PA2146. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Macrophage metalloelastase MMP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2146)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP12 (428-444aa KIDAVFYSKNKYYYFFQ).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2146 is reactive to MMP12 in Human

Applications

PA2146 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MMP12 (Source: Uniprot.Org, NCBI)

Uniprot ID

P39900

Gene ID

4321

Gene Name

MMP12

Full Name

Macrophage metalloelastase

Weight

54.002kDa

Superfamily

peptidase M10A family

Alternative Names

EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP12, check out the MMP12 Infographic

MMP12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-Macrophage metalloelastase MMP12 Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-MMP12 antibody PA2146. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-06

Question

Do you have a BSA free version of anti-MMP12 antibody PA2146 available?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MMP12 antibody PA2146 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-24

Question

We have seen staining in human amniotic fluid. Any tips? Is anti-MMP12 antibody supposed to stain amniotic fluid positively?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

From literature amniotic fluid does express MMP12. From Uniprot.org, MMP12 is expressed in amniotic fluid, alveolar macrophage, esophagus, among other tissues. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-16

Question

Does PA2146 anti-MMP12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

As indicated on the product datasheet, PA2146 anti-MMP12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-31

Question

I was wanting to use using your anti-MMP12 antibody for negative regulation of type i interferon-mediated signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

Thanks for your inquiry. This PA2146 anti-MMP12 antibody is tested on smmc cell lysate, hepa cell lysate, hela cell lysate, k562 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-17

Question

I see that the anti-MMP12 antibody PA2146 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-02

Question

I was wanting to use your anti-MMP12 antibody for WB for human amniotic fluid on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amniotic fluid identification?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

As indicated on the product datasheet, PA2146 anti-MMP12 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human amniotic fluid in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-02

Question

See attached the WB image, lot number and protocol we used for amniotic fluid using anti-MMP12 antibody PA2146. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-01

Question

Is this PA2146 anti-MMP12 antibody reactive to the isotypes of MMP12?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

The immunogen of PA2146 anti-MMP12 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP12(428-444aa KIDAVFYSKNKYYYFFQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-28

Question

Is a blocking peptide available for product anti-MMP12 antibody (PA2146)?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

We do provide the blocking peptide for product anti-MMP12 antibody (PA2146). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-28

Question

My boss were satisfied with the WB result of your anti-MMP12 antibody. However we have seen positive staining in amniotic fluid secreted using this antibody. Is that expected? Could you tell me where is MMP12 supposed to be expressed?

J. Thomas

Verified customer

Asked: 2018-07-31

Answer

Based on literature, amniotic fluid does express MMP12. Generally MMP12 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-07-31

Question

I am interested in to test anti-MMP12 antibody PA2146 on human amniotic fluid for research purposes, then I may be interested in using anti-MMP12 antibody PA2146 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-10-11

Answer

The products we sell, including anti-MMP12 antibody PA2146, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-10-11

Question

I have a question about product PA2146, anti-MMP12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-07-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2146 anti-MMP12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-07-20

Question

We are currently using anti-MMP12 antibody PA2146 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

J. Jha

Verified customer

Asked: 2016-08-09

Answer

The anti-MMP12 antibody (PA2146) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-09

Question

Will anti-MMP12 antibody PA2146 work for WB with amniotic fluid?

Z. Dhar

Verified customer

Asked: 2014-01-23

Answer

According to the expression profile of amniotic fluid, MMP12 is highly expressed in amniotic fluid. So, it is likely that anti-MMP12 antibody PA2146 will work for WB with amniotic fluid.

Boster Scientific Support

Answered: 2014-01-23

Size

Total: $315

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA2146
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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