Anti-SP1 Antibody Picoband™

SP1 antibody

Boster Bio Anti-SP1 Antibody Picoband™ catalog # A00110-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00110-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC, ICC, WB

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Product Name

Anti-SP1 Antibody Picoband™

View all SP1 Antibodies

SKU/Catalog Number

A00110-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SP1 Antibody Picoband™ catalog # A00110-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00110-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SP1 recombinant protein (Position: Q384-A603).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00110-1 is reactive to SP1 in Human, Mouse, Rat

Applications

A00110-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

80.693kDa

Background of SP1

Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SP1 (Source: Uniprot.org, NCBI)

Gene Name

SP1

Full Name

Transcription factor Sp1

Weight

80.693kDa

Superfamily

Sp1 C2H2-type zinc-finger protein family

Alternative Names

Sp1 transcription factor; specificity protein 1; transcription factor Sp1; TSFP1 SP1 Sp1 transcription factor transcription factor Sp1|specificity protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SP1, check out the SP1 Infographic

SP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00110-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Vascular endothelial growth factor induces multidrug resistance-associated protein 1 overexpression through phosphatidylinositol-3-kinase /protein kinase B signaling pathway and transcription factor specificity protein 1 in BGC823 cell line

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

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Have a question?

Find answers in Q&As, reviews.

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17 Customer Q&As for Anti-SP1 Antibody Picoband™

Question

We have observed staining in rat nipple. Do you have any suggestions? Is anti-SP1 antibody supposed to stain nipple positively?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

According to literature nipple does express SP1. According to Uniprot.org, SP1 is expressed in nipple, brain testis, cervix carcinoma, colon, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SP1 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 10973950, 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 10976766

Boster Scientific Support

Answered: 2020-04-24

Question

Will anti-SP1 antibody A00110-1 work for ICC with brain testis?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

According to the expression profile of brain testis, SP1 is highly expressed in brain testis. So, it is likely that anti-SP1 antibody A00110-1 will work for ICC with brain testis.

Boster Scientific Support

Answered: 2019-11-14

Question

I was wanting to use your anti-SP1 antibody for ICC for rat brain testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain testis identification?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

As indicated on the product datasheet, A00110-1 anti-SP1 antibody has been validated for ELISA, IF, IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-16

Question

Can you help my question with product A00110-1, anti-SP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00110-1 anti-SP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-15

Question

Will A00110-1 anti-SP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Gonzalez

Verified customer

Asked: 2019-10-02

Answer

You can see on the product datasheet, A00110-1 anti-SP1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-02

Question

Does anti-SP1 antibody A00110-1 work on feline IHC-P with brain testis?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

Our lab technicians have not validated anti-SP1 antibody A00110-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-SP1 antibody A00110-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain testis in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-01

Question

Our team were happy with the WB result of your anti-SP1 antibody. However we have been able to see positive staining in nipple nucleus. cytoplasm. note=nuclear location is using this antibody. Is that expected? Could you tell me where is SP1 supposed to be expressed?

A. Rodriguez

Verified customer

Asked: 2019-09-10

Answer

According to literature, nipple does express SP1. Generally SP1 expresses in nucleus. cytoplasm. note=nuclear location is. Regarding which tissues have SP1 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 10973950, 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 10976766

Boster Scientific Support

Answered: 2019-09-10

Question

I see that the anti-SP1 antibody A00110-1 works with ICC, what is the protocol used to produce the result images on the product page?

B. Banerjee

Verified customer

Asked: 2019-08-19

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-19

Question

Do you have a BSA free version of anti-SP1 antibody A00110-1 available?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SP1 antibody A00110-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-09

Question

Is this A00110-1 anti-SP1 antibody reactive to the isotypes of SP1?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

The immunogen of A00110-1 anti-SP1 antibody is E. coli-derived human SP1 recombinant protein (Position: Q384-A603). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-24

Question

Here is the WB image, lot number and protocol we used for brain testis using anti-SP1 antibody A00110-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-17

Question

We are currently using anti-SP1 antibody A00110-1 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-05

Answer

The anti-SP1 antibody (A00110-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-05

Question

Is a blocking peptide available for product anti-SP1 antibody (A00110-1)?

Verified Customer

Verified customer

Asked: 2019-01-10

Answer

We do provide the blocking peptide for product anti-SP1 antibody (A00110-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-10

Question

We need to test anti-SP1 antibody A00110-1 on rat brain testis for research purposes, then I may be interested in using anti-SP1 antibody A00110-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-06-26

Answer

The products we sell, including anti-SP1 antibody A00110-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-06-26

Question

We bought anti-SP1 antibody for ICC on brain testis in a previous experiment. I am using human, and We are going to use the antibody for WB next. I would like examining brain testis as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-09-28

Answer

I have checked the website and datasheets of our anti-SP1 antibody and I see that A00110-1 has been tested on human in both ICC and WB. Thus A00110-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-09-28

Question

you antibody using your anti-SP1 antibody for snrna transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

B. Evans

Verified customer

Asked: 2015-12-24

Answer

We appreciate your inquiry. This A00110-1 anti-SP1 antibody is validated on human tonsil tissue, k562 whole cell lysate, a549 whole cell lysate, hela whole cell lysate, a431 whole cell lysate, rat liver tissue, brain tissue, mouse liver tissue, intestinal cancer tissue, lung cancer tissue, squama cancer tissue, ovary cancer tissue, spleen tissue, small intestine tissue. It is guaranteed to work for ELISA, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-12-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain testis using anti-SP1 antibody A00110-1. Let me know if you need anything else.

T. Krishna

Verified customer

Asked: 2014-05-21

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-05-21

Order DetailsPrice
A00110-1

100μg

$370
A00110-1-10ug

10μg sample (liquid)

$99
A00110-1-Biotin

100 μg Biotin conjugated

$570
A00110-1-Cy3

100 μg Cy3 conjugated

$570
A00110-1-Dylight488

100 μg Dylight488 conjugated

$570
A00110-1-Dylight550

100 μg Dylight550 conjugated

$570
A00110-1-Dylight594

100 μg Dylight594 conjugated

$570
A00110-1-FITC

100 μg FITC conjugated

$570
A00110-1-HRP

100 μg HRP conjugated

$570
A00110-1-APC

100 μg APC conjugated

$670
A00110-1-PE

100 μg PE conjugated

$670
A00110-1-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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