Product Info Summary
SKU: | PB9458 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Vitronectin/VTN Antibody Picoband™
View all Vitronectin Antibodies
SKU/Catalog Number
PB9458
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vitronectin/VTN Antibody Picoband™ catalog # PB9458. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitronectin/VTN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9458)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Vitronectin, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9458 is reactive to VTN in Human, Mouse, Rat
Applications
PB9458 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.306kDa
Background of Vitronectin
Vitronectin, also known as VTN, is a protein that in humans is encoded by the VTN gene. The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Also vitronectin serves to regulate proteolysis initiated by plasminogen activation. In addition, vitronectin is a component of platelets and is, thus, involved in hemostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9458).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Rat Lung Tissue Lysate at 50ug,
Lane 3: HEPG2 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug,
Lane 5: HEPA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9458) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 54 kDa. The expected band size for Vitronectin is at 54 kDa.
Click image to see more details
Figure 2. IHC analysis of Vitronectin using anti-Vitronectin antibody (PB9458).
Vitronectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Vitronectin Antibody (PB9458) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For VTN (Source: Uniprot.org, NCBI)
Gene Name
VTN
Full Name
Vitronectin
Weight
54.306kDa
Alternative Names
Complement S-protein; epibolin; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; S-protein; V75; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin; VN; VNT; VTN VTN V75, VN, VNT vitronectin vitronectin|complement S-protein|epibolin|serum spreading factor|somatomedin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VTN, check out the VTN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vitronectin/VTN Antibody Picoband™ (PB9458)
Hello CJ!
PB9458 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang J,Li Y,Pan L,Li J,Yu Y,Liu B,Zubair M,Wei Y,Pillay B,Olaniran AO,Chiliza TE,Shao G,Feng Z,Xiong Q.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation.Vet Res.2021 Jun 3;52(1):80.doi:10.1186/s13567-021-00952-8. PMID:34082810;PMCID:PMC8173509.
Species: Human
PB9458 usage in article: APP:WB, SAMPLE:M. HYORHINIS CELL, DILUTION:1:2000
Autocrine fibronectin from differentiating mesenchymal stem cells induces the neurite elongation in vitro and promotes nerve fiber regeneration in transected spinal cord injury
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Vitronectin/VTN Antibody Picoband™
Question
We are currently using anti-Vitronectin/VTN antibody PB9458 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
B. Gonzalez
Verified customer
Asked: 2017-10-23
Answer
The anti-Vitronectin/VTN antibody (PB9458) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-23