Anti-SQSTM1/p62 Antibody Picoband™

p62/SQSTM1 antibody

Boster Bio Anti-SQSTM1/p62 Antibody Picoband™ catalog # PB9444. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9444
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-SQSTM1/p62 Antibody Picoband™

View all p62/SQSTM1 Antibodies

SKU/Catalog Number

PB9444

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SQSTM1/p62 Antibody Picoband™ catalog # PB9444. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SQSTM1/p62 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9444)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9444 is reactive to SQSTM1 in Human, Mouse, Rat

Applications

PB9444 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

62 kDa

Calculated molecular weight

47.687kDa

Background of p62/SQSTM1

SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 2μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For SQSTM1 (Source: Uniprot.org, NCBI)

Gene Name

SQSTM1

Full Name

Sequestosome-1

Weight

47.687kDa

Alternative Names

A170; Autophagy Receptor P62; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; ORCA; OSIL; oxidative stress induced like; p60PDB3; p62; p62B; Paget disease of bone 3; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome 1; sequestosome-1; SQSTM1; Ubiquitin-binding protein p62; ZIP3 SQSTM1 A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B sequestosome 1 sequestosome-1|EBI3-associated protein of 60 kDa|EBI3-associated protein p60|EBIAP|autophagy receptor p62|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa|ubiquitin-binding protein p62

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SQSTM1, check out the SQSTM1 Infographic

SQSTM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SQSTM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9444 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Oleuropein Induces AMPK-Dependent Autophagy in NAFLD Mice, Regardless of the Gender

Zhang Tao,Xiaoqing Zhou,Yan Zhang,Wenfeng Pu,Yi Yang,Fuxia Wei,Qian Zhou,Lin Zhang,Zhonghan Du,Ji Wu,"Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-α-Stimulated Colitis in CACO2 Cells: Involvement of the NLRP3 Inflammasome and Autophagy",Mediators of Inflammation,vol. 2021, Article ID 1610251,12 pages,2021.https://doi.org/10.1155/2021/1610251
Species: Human,Rat
PB9444 usage in article: APP:WB, SAMPLE:COLON TISSUE AND CACO2 CELL, DILUTION:1:1500

Liu, J.,Qin, X.,Ma, W.,Jia, S.,Zhang, X.,Yang, X. ... Jin, F. (2021). Corilagin induces apoptosis and autophagy in NRF2‑addicted U251 glioma cell line. Molecular Medicine Reports,23,320. https://doi.org/10.3892/mmr.2021.11959
Species: Human
PB9444 usage in article: APP:WB, SAMPLE:U251 CELL, DILUTION:1:1000

Zhong W,Tang X,Liu Y,Zhou C,Liu P,Li E,Zhong P,Lv H,Zou Q,Wang M.Benzoxazole Derivative K313 Induces Cell Cycle Arrest, Apoptosis and Autophagy Blockage and Suppresses mTOR/p70S6K Pathway in Nalm-6 and Daudi Cells.Molecules.2020 Feb 21;25(4):971.doi:10.33
Species: Human
PB9444 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA

Liu X,Feng C,Wei G,Kong W,Meng H,Du Y,Li J.Mitofusin1 Is a Major Mediator in Glucose-Induced Epithelial-to-Mesenchymal Transition in Lung Adenocarcinoma Cells.Onco Targets Ther.2020 Apr 24;13:3511-3523.doi:10.2147/OTT.S238714.PMID:32425551;PMCID:PMC718794
Species: Human
PB9444 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:500

Yuan H,Wang Y,Chen H,Cai X.Protective effect of flavonoids from Rosa roxburghii Tratt on myocardial cells via autophagy.3 Biotech.2020 Feb;10(2):58.doi:10.1007/s13205-019-2049-1.Epub 2020 Jan 22.PMID: 32015954;PMCID:PMC6976074.
Species: Rat
PB9444 usage in article: APP:IF, SAMPLE:RAT MYOCARDIAL CELLS, DILUTION:1:500

Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human

Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage

Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice

Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta

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16 Customer Q&As for Anti-SQSTM1/p62 Antibody Picoband™

Question

I have attached the WB image, lot number and protocol we used for caudate nucleus trachea using anti-SQSTM1/p62 antibody PB9444. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-30

Question

We are currently using anti-SQSTM1/p62 antibody PB9444 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

The anti-SQSTM1/p62 antibody (PB9444) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-21

Question

you antibody using your anti-SQSTM1/p62 antibody for negative regulation of transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

We appreciate your inquiry. This PB9444 anti-SQSTM1/p62 antibody is validated on human placenta tissue, intestine tissue, small intestine tissue, a549 whole cell lysates, u87 whole cell lysates, hela whole cell lysates, hepg2 whole cell lysates, rat rh35 whole cell lysates. It is guaranteed to work for IF, IHC, ICC, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-12

Question

I was wanting to use your anti-SQSTM1/p62 antibody for ICC for monkey caudate nucleus trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for monkey caudate nucleus trachea identification?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

It shows on the product datasheet, PB9444 anti-SQSTM1/p62 antibody has been tested for IF, IHC, ICC, WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in monkey caudate nucleus trachea in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-10

Question

We bought anti-SQSTM1/p62 antibody for WB on placenta a few months ago. I am using monkey, and I plan to use the antibody for IHC next. Our lab want to know about examining placenta as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

I looked at the website and datasheets of our anti-SQSTM1/p62 antibody and it appears that PB9444 has been tested on monkey in both WB and IHC. Thus PB9444 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in monkey even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-09

Question

Would anti-SQSTM1/p62 antibody PB9444 work for ICC with caudate nucleus trachea?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

According to the expression profile of caudate nucleus trachea, SQSTM1 is highly expressed in caudate nucleus trachea. So, it is likely that anti-SQSTM1/p62 antibody PB9444 will work for ICC with caudate nucleus trachea.

Boster Scientific Support

Answered: 2019-07-24

Question

I am looking for to test anti-SQSTM1/p62 antibody PB9444 on monkey caudate nucleus trachea for research purposes, then I may be interested in using anti-SQSTM1/p62 antibody PB9444 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

The products we sell, including anti-SQSTM1/p62 antibody PB9444, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-08

Question

My colleagues were well pleased with the WB result of your anti-SQSTM1/p62 antibody. However we have seen positive staining in leukemic t-cell cytosol using this antibody. Is that expected? Could you tell me where is SQSTM1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

According to literature, leukemic t-cell does express SQSTM1. Generally SQSTM1 expresses in cytoplasm, cytosol. Regarding which tissues have SQSTM1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8551575
Caudate nucleus, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 8650207, 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Pancreas, Placenta, Skin, and Uterus, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-03-15

Question

I see that the anti-SQSTM1/p62 antibody PB9444 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-19

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-19

Question

Do you have a BSA free version of anti-SQSTM1/p62 antibody PB9444 available?

Verified Customer

Verified customer

Asked: 2018-12-24

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SQSTM1/p62 antibody PB9444 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-24

Question

I have a question about product PB9444, anti-SQSTM1/p62 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Parker

Verified customer

Asked: 2018-09-24

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9444 anti-SQSTM1/p62 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-24

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus trachea using anti-SQSTM1/p62 antibody PB9444. Let me know if you need anything else.

N. Krishna

Verified customer

Asked: 2017-12-05

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-05

Question

We have seen staining in mouse cervix carcinoma. Any tips? Is anti-SQSTM1/p62 antibody supposed to stain cervix carcinoma positively?

E. Mangal

Verified customer

Asked: 2016-10-04

Answer

From what I have seen in literature cervix carcinoma does express SQSTM1. From what I have seen in Uniprot.org, SQSTM1 is expressed in left adrenal gland cortex, b-cell, cervix carcinoma, caudate nucleus trachea, pancreas, placenta, skin uterus, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SQSTM1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8551575
Caudate nucleus, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 8650207, 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Pancreas, Placenta, Skin, and Uterus, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-10-04

Question

Does PB9444 anti-SQSTM1/p62 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Brown

Verified customer

Asked: 2016-03-10

Answer

It shows on the product datasheet, PB9444 anti-SQSTM1/p62 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-03-10

Question

Is a blocking peptide available for product anti-SQSTM1/p62 antibody (PB9444)?

J. Krishna

Verified customer

Asked: 2015-11-04

Answer

We do provide the blocking peptide for product anti-SQSTM1/p62 antibody (PB9444). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-11-04

Question

Is this PB9444 anti-SQSTM1/p62 antibody reactive to the isotypes of SQSTM1?

B. Yang

Verified customer

Asked: 2014-02-27

Answer

The immunogen of PB9444 anti-SQSTM1/p62 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62 (69-96aa DEDGDLVAFSSDEELTMAMSYVKDDIFR), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-02-27

Order DetailsPrice
PB9444

100μg

$370
PB9444-10ug

10μg sample (liquid)

$99
PB9444-Biotin

100 μg Biotin conjugated

$570
PB9444-Cy3

100 μg Cy3 conjugated

$570
PB9444-Dylight488

100 μg Dylight488 conjugated

$570
PB9444-Dylight550

100 μg Dylight550 conjugated

$570
PB9444-Dylight594

100 μg Dylight594 conjugated

$570
PB9444-FITC

100 μg FITC conjugated

$570
PB9444-HRP

100 μg HRP conjugated

$570
PB9444-APC

100 μg APC conjugated

$670
PB9444-PE

100 μg PE conjugated

$670
PB9444-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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