Product Info Summary
| SKU: | A03473 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Steroid hormone receptor ERR2 ESRRB Antibody
SKU/Catalog Number
A03473
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Steroid hormone receptor ERR2 ESRRB Antibody (Catalog # A03473). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
ESRRB antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Steroid hormone receptor ERR2 ESRRB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03473)
Host
Rabbit
Contents
ESRRB Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ESRRB antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ESRRB. The immunogen is located within amino acids 360 - 410 of ESRRB.
Reactive Species
A03473 is reactive to ESRRB in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.1 kDa
Background of ESRRB
The estrogen-related receptor beta (ESRRB) was initially identified in mouse embryonic carcinoma stem cells and was found to be expressed exclusively in trophoblast progenitor cells between days 6.5 and 7.5 post coitum. Recent studies have shown that ESRRB is part of an POU5F1/Oct4-centered protein interaction network in embryonic stem (ES) cells. It is thought to interact with other ES transcription factors such as NANOG within this network, activating POU5F1 transcription and thereby sustaining self-renewal and pluripotency in ES cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03473 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ESRRB antibody can be used for detection of ESRRB by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ESRRB in human heart tissue lysate with ESRRB antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ESRR8 in human heart tissue with ESRR8 antibody at 5 μg/mL.
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Immunofluorescence of ESRRB in human heart tissue with ESRRB antibody at 20 μg/mL.
Specific Publications For Anti-Steroid hormone receptor ERR2 ESRRB Antibody (A03473)
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