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Product Info Summary
| SKU: | A03678 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-SURF-1 Antibody
SKU/Catalog Number
A03678
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SURF-1 Antibody catalog # A03678. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SURF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03678)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SURF1. AA range:171-220
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03678 is reactive to SURF1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
33.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03678 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of Jurkat cells using SURF-1 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using SURF1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-SURF-1 Antibody (A03678)
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Customer Q&As
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5 Customer Q&As for Anti-SURF-1 Antibody
Question
Will anti-SURF-1 antibody A03678 work on pig WB with kidney?
K. Moore
Verified customer
Asked: 2020-01-10
Answer
Our lab technicians have not validated anti-SURF-1 antibody A03678 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SURF-1 antibody A03678 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-10
Question
I was wanting to use your anti-SURF-1 antibody for WB for rat parotid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat parotid gland identification?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can see on the product datasheet, A03678 anti-SURF-1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat parotid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-01
Question
Is a blocking peptide available for product anti-SURF-1 antibody (A03678)?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
We do provide the blocking peptide for product anti-SURF-1 antibody (A03678). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-17
Question
We are currently using anti-SURF-1 antibody A03678 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
D. Evans
Verified customer
Asked: 2014-08-18
Answer
The anti-SURF-1 antibody (A03678) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-18
Question
Will anti-SURF-1 antibody A03678 work for WB with parotid gland?
S. Krishna
Verified customer
Asked: 2014-04-14
Answer
According to the expression profile of parotid gland, SURF1 is highly expressed in parotid gland. So, it is likely that anti-SURF-1 antibody A03678 will work for WB with parotid gland.
Boster Scientific Support
Answered: 2014-04-14
