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- Table of Contents
SYP antibody (M05049) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Immunofluorescent analysis of PC-12 cells, using Synaptophysin Antibody .
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Anti-Synaptophysin SYP Rabbit Monoclonal Antibody
SYP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
SYP antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SYP antibody was positively detected by IHC in:
human colon tissue, human pancreas tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SYP antibody was positively detected by ICC/IF in:
PC-12 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Human
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Synaptophysin SYP Rabbit Monoclonal Antibody
View all SYP Antibodies
View all SYP Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BIE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Synaptophysin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
33.8 kDa
Cite This Product
Anti-Synaptophysin SYP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05049)
Protocols
Western Blot protocol for SYP antibody
Immunohistochemistry (IHC) protocol for SYP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SYP antibody
Publications
Application:
Species:
Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance
Published: 2024-01-15
Journal: MOLECULAR NEUROBIOLOGY
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 38221533
Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral ischemia in rats
Published: 2009-06-09
Journal: BRAIN RESEARCH
Application: IHC
Impact Factor: 2.49
Species: Rat
PMID: 19520066
Review
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Product Promise
We promise Anti-Synaptophysin SYP Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M05049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026