Anti-Synaptophysin SYP Rabbit Monoclonal Antibody

Boster Bio Anti-Synaptophysin SYP Rabbit Monoclonal Antibody catalog # M05049. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M05049
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-Synaptophysin SYP Rabbit Monoclonal Antibody

See all Synaptophysin products

SKU/Catalog Number

M05049

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Synaptophysin SYP Rabbit Monoclonal Antibody catalog # M05049. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Synaptophysin SYP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05049)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BIE-19

Immunogen

A synthesized peptide derived from human Synaptophysin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M05049 is reactive to SYP in Human, Mouse, Rat

Applications

M05049 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Flow Cytometry 1:50

Validation Images & Assay Conditions

Gene/Protein Information For SYP (Source: Uniprot.Org, NCBI)

Uniprot ID

P08247

Gene ID

6855

Gene Name

SYP

Full Name

Synaptophysin

Weight

33.845kDa

Superfamily

synaptophysin/synaptobrevin family

Alternative Names

Major synaptic vesicle protein p38; MRX; MRXSYP; Synaptophysin; SYP SYP MRX96, MRXSYP synaptophysin synaptophysin|major synaptic vesicle protein P38

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SYP, check out the SYP Infographic

SYP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SYP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M05049 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lin J,Hao C,Gong Y,Zhang Y,Li Y,Feng Z,Xu X,Huang H,Liao W. Effect of Tetramethylpyrazine on Neuroplasticity after Transient Focal Cerebral Ischemia Reperfusion in Rats. Evid Based Complement Alternat Med. 2021 Jan 18;2021:1587241.doi:10.1155/2021/1587241
Species: Rat
M05049 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L. Neural Regen Res. 2013 Oct 15;8(29):2725-33. Doi: 10.3969/J.Issn.1673-5374.2013.29.004. Downregulation Of Caveolin-1 Contributes To The Synaptic Plasticity Deficit In The Hippocampus Of Aged Rats.

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16 Customer Q&As for Anti-Synaptophysin SYP Rabbit Monoclonal Antibody

Question

We bought anti-Synaptophysin Rabbit Monoclonal antibody for IF on amygdala in a previous project. I am using rat, and We want to use the antibody for WB next. We need examining amygdala as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

I have checked the website and datasheets of our anti-Synaptophysin Rabbit Monoclonal antibody and I see that M05049 has been tested on rat in both IF and WB. Thus M05049 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-17

Question

We have observed staining in rat amygdala. What should we do? Is anti-Synaptophysin Rabbit Monoclonal antibody supposed to stain amygdala positively?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

From literature amygdala does express SYP. From Uniprot.org, SYP is expressed in anterior cingulate cortex, amygdala, brain, among other tissues. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-04

Question

Is a blocking peptide available for product anti-Synaptophysin Rabbit Monoclonal antibody (M05049)?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

We do provide the blocking peptide for product anti-Synaptophysin Rabbit Monoclonal antibody (M05049). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-27

Question

I am interested in using your anti-Synaptophysin Rabbit Monoclonal antibody for synaptic vesicle membrane organization studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

We appreciate your inquiry. This M05049 anti-Synaptophysin Rabbit Monoclonal antibody is tested on rat brain tissue, mouse brain. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin Rabbit Monoclonal antibody M05049. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-22

Question

We are currently using anti-Synaptophysin Rabbit Monoclonal antibody M05049 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The anti-Synaptophysin Rabbit Monoclonal antibody (M05049) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-30

Question

I see that the anti-Synaptophysin Rabbit Monoclonal antibody M05049 works with IHC, what is the protocol used to produce the result images on the product page?

R. Krishna

Verified customer

Asked: 2019-09-20

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-20

Question

See attached the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin Rabbit Monoclonal antibody M05049. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-09

Question

Do you have a BSA free version of anti-Synaptophysin Rabbit Monoclonal antibody M05049 available?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Synaptophysin Rabbit Monoclonal antibody M05049 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-22

Question

Is this M05049 anti-Synaptophysin Rabbit Monoclonal antibody reactive to the isotypes of SYP?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

The immunogen of M05049 anti-Synaptophysin Rabbit Monoclonal antibody is A synthesized peptide derived from human Synaptophysin . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-21

Question

Would M05049 anti-Synaptophysin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-13

Answer

You can see on the product datasheet, M05049 anti-Synaptophysin Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-13

Question

My question regarding product M05049, anti-Synaptophysin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-24

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05049 anti-Synaptophysin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-24

Question

Does anti-Synaptophysin Rabbit Monoclonal antibody M05049 work for IHC with anterior cingulate cortex?

Verified Customer

Verified customer

Asked: 2017-12-12

Answer

According to the expression profile of anterior cingulate cortex, SYP is highly expressed in anterior cingulate cortex. So, it is likely that anti-Synaptophysin Rabbit Monoclonal antibody M05049 will work for IHC with anterior cingulate cortex.

Boster Scientific Support

Answered: 2017-12-12

Question

My team were satisfied with the WB result of your anti-Synaptophysin Rabbit Monoclonal antibody. However we have been able to see positive staining in amygdala cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYP supposed to be expressed?

M. Jackson

Verified customer

Asked: 2017-01-27

Answer

According to literature, amygdala does express SYP. Generally SYP expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-01-27

Question

I was wanting to use your anti-Synaptophysin Rabbit Monoclonal antibody for IHC for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse anterior cingulate cortex identification?

P. Patel

Verified customer

Asked: 2016-05-06

Answer

You can see on the product datasheet, M05049 anti-Synaptophysin Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-05-06

Question

I was wanting to use to test anti-Synaptophysin Rabbit Monoclonal antibody M05049 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-Synaptophysin Rabbit Monoclonal antibody M05049 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Zhao

Verified customer

Asked: 2014-05-19

Answer

The products we sell, including anti-Synaptophysin Rabbit Monoclonal antibody M05049, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-05-19

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M05049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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