This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
TCP1 antibody (M02389) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of HepG2 cells using anti-TCP1 alpha antibody (M02389).
Overlay histogram showing HepG2 cells stained with M02389 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TCP1 alpha Antibody (M02389,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HepG2 cells stained with M02389 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TCP1 alpha Antibody (M02389,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Bundle Save
Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)
TCP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TCP1 antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human COLO-320 whole cell, human HepG2 whole cell, human A431 whole cell, human HT1080 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TCP1 antibody was positively detected by IHC in:
human lung cancer tissue, human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TCP1 antibody was positively detected by ICC/IF in:
MCF7 cell
TCP1 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)
View all TCP1 Antibodies
View all TCP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2E7
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TCP1 alpha, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
60.3 kDa
Background of TCP1
T-complex protein 1 subunit alpha is a protein that in humans is encoded by the TCP1 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.
Cite This Product
Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02389)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
97%
High Homology
Cow
97%
High Homology
Sheep
97%
High Homology
Dog
97%
High Homology
Rat
95%
High Homology
Rabbit
95%
High Homology
Zebrafish
73%
Moderate Homology
Drosophila
70%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TCP1 antibody
Immunohistochemistry (IHC) protocol for TCP1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TCP1 antibody
Flow Cytometry (FC) protocol for TCP1 antibody
Publications
Application:
Species:
CCT2 is an aggrephagy receptor for clearance of solid protein aggregates
Published: 2022-04-01
Journal: CELL
Application: WB
Impact Factor: 66.85
Species: Human
PMID: 35366418
Review
Have you used Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)
In this study, these two antibodies were primarily used for Western blot experiments. In the Western blot results, the CCT1 antibody showed a strong specific band and maintained a robust signal even after multiple reuses.
| SKU | M02389 |
|---|---|
| Application | Western Blot |
| Sample | U2OS cell |
| Sample Processing Description | Cells were lysed with NP-40 lysis buffer to extract proteins. After quantification using the BCA assay, 5× loading buffer was added, and the samples were boiled for denaturation. Then, 20 µg of protein was loaded into each lane. |
| Primary Antibody | Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | BSA |
| Secondary Antibody | Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Unconjugated (BA1039) |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: ChemiDoc MP (Bio-Rad) |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
Xinyu Ma, Tsinghua University
Verified customer
Submitted 2025-10-27
Product Promise
We promise Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02389
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026