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Product Info Summary
| SKU: | PB9023 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband®
SKU/Catalog Number
PB9023
PB0023 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband® catalog # PB9023. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9023)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCL18 recombinant protein (Position: A21-A89).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9023 is reactive to CCL18 in Human
Observed Molecular Weight
19 kDa
Calculated molecular weight
9.8 kDa
Background of CCL18
Macrophage Inflammatory Protein 4, also known as CCL18, is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9023 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA(Cap) , 1-5μg/ml, Human
Validation Images & Assay Conditions
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Western blot analysis of CCL18 using anti-CCL18 antibody (PB9023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human CCL18 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL18 antigen affinity purified polyclonal antibody (Catalog # PB9023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL18 at approximately 19KD. The expected band size for CCL18 is at 19KD.
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Sandwich ELISA - Recombinant human CCL18/PARC protein standard curve.
Use in combination with reagents from Human CCL18/PARC ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0686).
Specific Publications For Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband® (PB9023)
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Customer Q&As
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4 Customer Q&As for Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband®
Question
I have a question about product PB9023, anti-Macrophage Inflammatory Protein 4/CCL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9023 anti-Macrophage Inflammatory Protein 4/CCL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-02
Question
Is a blocking peptide available for product anti-Macrophage Inflammatory Protein 4/CCL18 antibody (PB9023)?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
We do provide the blocking peptide for product anti-Macrophage Inflammatory Protein 4/CCL18 antibody (PB9023). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-22
Question
Would PB9023 anti-Macrophage Inflammatory Protein 4/CCL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
It shows on the product datasheet, PB9023 anti-Macrophage Inflammatory Protein 4/CCL18 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-18
Question
We are currently using anti-Macrophage Inflammatory Protein 4/CCL18 antibody PB9023 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
A. Carter
Verified customer
Asked: 2014-11-19
Answer
The anti-Macrophage Inflammatory Protein 4/CCL18 antibody (PB9023) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-19
