Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™

CCL18/PARC antibody

Boster Bio Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™ catalog # PB9023. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PB9023
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™

View all CCL18/PARC Antibodies

SKU/Catalog Number

PB9023

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™ catalog # PB9023. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9023)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CCL18 recombinant protein (Position: A21-A89).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9023 is reactive to CCL18 in Human

Applications

PB9023 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

9.849kDa

Background of CCL18/PARC

Macrophage Inflammatory Protein 4, also known as CCL18, is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA(Cap) , 1-5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CCL18 (Source: Uniprot.org, NCBI)

Gene Name

CCL18

Full Name

C-C motif chemokine 18

Weight

9.849kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated CCL18 AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18 C-C motif chemokine ligand 18 C-C motif chemokine 18|CC chemokine PARC|CC chemokine ligand 18|alternative macrophage activation-associated CC chemokine 1|chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)|chemokine (C-C), dendritic|dendritic cell chemokine 1|macrophage inflammatory protein 4|pulmonary and activation-regulated chemokine|small inducible cytokine A18|small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCL18, check out the CCL18 Infographic

CCL18 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9023 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Blum L, Ulshöfer T, Henke M, Krieg R, Berneburg I, Geisslinger G, Becker K, Parnham MJ, Schiffmann S. The immunomodulatory potential of the arylmethylaminosteroid sc1o. J Mol Med (Berl). 2020 Dec 17. doi: 10.1007/s00109-020-02024-4. Epub ahead of print. P
Species: Human
PB9023 usage in article: APP:ELISA, SAMPLE:DENDRITIC CELL, DILUTION:NA

Have you used Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband™

Question

I have a question about product PB9023, anti-Macrophage Inflammatory Protein 4/CCL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9023 anti-Macrophage Inflammatory Protein 4/CCL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-02

Question

Is a blocking peptide available for product anti-Macrophage Inflammatory Protein 4/CCL18 antibody (PB9023)?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

We do provide the blocking peptide for product anti-Macrophage Inflammatory Protein 4/CCL18 antibody (PB9023). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-22

Question

Would PB9023 anti-Macrophage Inflammatory Protein 4/CCL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-10-18

Answer

It shows on the product datasheet, PB9023 anti-Macrophage Inflammatory Protein 4/CCL18 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-18

Question

We are currently using anti-Macrophage Inflammatory Protein 4/CCL18 antibody PB9023 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

A. Carter

Verified customer

Asked: 2014-11-19

Answer

The anti-Macrophage Inflammatory Protein 4/CCL18 antibody (PB9023) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-11-19

Order DetailsPrice
PB9023

100μg

$370
PB9023-10ug

10μg sample (liquid)

$99
PB9023-Biotin

100 μg Biotin conjugated

$570
PB9023-Cy3

100 μg Cy3 conjugated

$570
PB9023-Dylight488

100 μg Dylight488 conjugated

$570
PB9023-Dylight550

100 μg Dylight550 conjugated

$570
PB9023-Dylight594

100 μg Dylight594 conjugated

$570
PB9023-FITC

100 μg FITC conjugated

$570
PB9023-HRP

100 μg HRP conjugated

$570
PB9023-APC

100 μg APC conjugated

$670
PB9023-PE

100 μg PE conjugated

$670
PB9023-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PB9023
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.