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Product Info Summary
| SKU: | PB9123 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-AAMP Antibody Picoband®
SKU/Catalog Number
PB9123
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AAMP CCL18 Antibody Picoband® catalog # PB9123. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AAMP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9123)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AAMP recombinant protein (Position: E235-R434).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9123 is reactive to AAMP in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
46.8 kDa
Background of AAMP
AAMP, also known as Angio-associated, migratory cell protein, is a protein which in humans is encoded by the AAMP gene. It is mapped to 2q35. The gene product of AAMP is an immunoglobulin-type protein, which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. It has been demonstrated that an AAMP peptide containing the putative heparan sulfate-binding domain binds to heparin and mediates heparin-sensitive cell adhesion. AAMP plays a role in angiogenesis and cell migration. In smooth muscle cell migration, it may act through the RhoA pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9123 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human MCF-7 whole cell, human HepG2 whole cell, human CACO-2 whole cell, human 293T whole cell, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue
IHC: human testis cancer tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human 293T whole cell lysates.
Lane 5: rat heart tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AAMP at approximately 52 kDa. The expected band size for AAMP is at 47 kDa.
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IHC analysis of AAMP using anti-AAMP antibody (PB9123).
AAMP was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AAMP Antibody (PB9123) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Flow Cytometry analysis of MCF-7 cells using anti-AAMP antibody (PB9123).
Overlay histogram showing MCF-7 cells stained with PB9123 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AAMP Antibody (PB9123, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-AAMP Antibody Picoband® (PB9123)
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6 Customer Q&As for Anti-AAMP Antibody Picoband®
Question
Will anti-AAMP antibody PB9123 work for IHC with ovary skin?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
According to the expression profile of ovary skin, AAMP is highly expressed in ovary skin. So, it is likely that anti-AAMP antibody PB9123 will work for IHC with ovary skin.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-AAMP antibody PB9123 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-AAMP antibody (PB9123) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
Is there a BSA free version of anti-AAMP antibody PB9123 available?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AAMP antibody PB9123 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-19
Question
Is a blocking peptide available for product anti-AAMP antibody (PB9123)?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
We do provide the blocking peptide for product anti-AAMP antibody (PB9123). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary skin using anti-AAMP antibody PB9123. Let me know if you need anything else.
J. Li
Verified customer
Asked: 2017-12-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-18
Question
My question regards to test anti-AAMP antibody PB9123 on human ovary skin for research purposes, then I may be interested in using anti-AAMP antibody PB9123 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Carter
Verified customer
Asked: 2017-08-11
Answer
The products we sell, including anti-AAMP antibody PB9123, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-11
