Anti-Telomerase/TERT Antibody

SKU PA1696
Size 100μg/vial
Reactivity Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-Telomerase/TERT Antibody
SKU/Catalog Number PA1696
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Telomerase reverse transcriptase(TERT) detection. Tested with WB in Mouse;Rat.
Cite This Product Anti-Telomerase/TERT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1696)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse Telomerase(15-30aa RSRYREVWPLATFVRR), identical to the related rat sequence.
Reactivity Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti-Telomerase antibody, PA1696, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: Mouse Testis Tissue Lysate
Lane 5: Mouse Kidney Tissue Lysate
Lane 6: Mouse Heart Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O70372
Gene Name Tert
Protein Name Telomerase reverse transcriptase
Tissue Specificity High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain. .
Alternative Names Telomerase reverse transcriptase;2.7.7.49;Telomerase catalytic subunit;Tert;
Subcellular Localization Nucleus, nucleolus . Nucleus, nucleoplasm . Nucleus. Chromosome, telomere. Cytoplasm . Nucleus, PML body . Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress- mediated phosphorylation at Tyr-697. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT (By similarity). .
Molecular Weight 127978 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA- dependent extension of 3'-chromosomal termini with the 6- nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity). .
Research Areas Mouse, Rat

*You can search these to find other products in these research areas.
Background TERT(Telomerase reverse transcriptase), is a catalytic subunit of the enzyme telomerase. Its absence(usually as a result of a chromosomal mutation) is associated with the disorder Cri du chat. The TERT gene was mapped to chromosome 5p15.33T by Meyerson, M. Elomerase lengthens telomeres in DNA strands, thereby allowing senescent cells that would otherwise become postmitotic and undergo apoptosis to exceed the Hayflick limit and become potentially immortal, as is often the case with cancerous cells. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells, resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mice suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.

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Polyclonal antibody for TERT detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Mouse. TERT information: Molecular Weight: 127978 MW; Subcellular Localization: Nucleus, nucleolus . Nucleus, nucleoplasm . Nucleus. Chromosome, telomere. Cytoplasm . Nucleus, PML body . Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress- mediated phosphorylation at Tyr-697. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT (By similarity); Tissue Specificity: High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain.
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In stock
Order Product
PA1696
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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Publications

An immortalized steroidogenic goat granulosa cell line as a model system to study the effect of the endoplasmic reticulum (ER)-stress response on steroidogenesis
Qiu J, Li Gw, Sui Yf, Sun Yj, Huang Yy, Si Sy, Ge W. Acta Oncol. 2007;46(7):961-8. Immunization With Truncated Sequence Of Telomerase Reverse Transcriptase Induces A Specific Antitumor Response In Vivo.
Sheng R, Gu Zl, Xie Ml. Int J Cardiol. 2013 Jan 20;162(3):199-209. Doi: 10.1016/J.Ijcard.2011.07.083. Epub 2011 Oct 15. Epigallocatechin Gallate, The Major Component Of Polyphenols In Green Tea, Inhibits Telomere Attrition Mediated Cardiomyocyte A...
Yang S, Brindley Pj, Zeng Q, Li Y, Zhou J, Liu Y, Liu B, Cai L, Zeng T, Wei Q, Lan L, Mcmanus Dp. Mol Biochem Parasitol. 2010 Dec;174(2):109-16. Doi: 10.1016/J.Molbiopara.2010.07.007. Epub 2010 Aug 6. Transduction Of Schistosoma Japonicum Schistos...

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to telomerase reverse transcriptase antibody, tert antibody
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