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Product Info Summary
| SKU: | A07288-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-THEM2 ACOT13 Antibody
SKU/Catalog Number
A07288-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-THEM2 ACOT13 Antibody (Catalog # A07288-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
THEM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-THEM2 ACOT13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07288-1)
Host
Rabbit
Contents
THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2. The immunogen is located within the last 50 amino acids of THEM2.
Reactive Species
A07288-1 is reactive to ACOT13 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
15.0 kDa
Background of ACOT13
THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07288-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
THEM2 antibody can be used for detection of THEM2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 μg/mL.
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Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2.5 μg/mL.
Specific Publications For Anti-THEM2 ACOT13 Antibody (A07288-1)
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