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- Table of Contents
Fas antibody (A00055) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of RAW264.7 cells using anti-Fas antibody (A00055).
Overlay histogram showing RAW264.7 cells stained with A00055 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fas Antibody (A00055, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RAW264.7 cells stained with A00055 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fas Antibody (A00055, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for FAS? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the FAS Western Blot GuideBundle Save
Anti-Fas Antibody Picoband®
Fas antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Fas antibody was positively detected by WB in:
rat thymus tissue, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Fas antibody was positively detected by FC in:
RAW2647 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-Fas Antibody Picoband®
View all FAS Antibodies
View all FAS Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Fas recombinant protein (Position: E46-K279).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated Molecular Weight
37.4 kDa
Background of Fas
FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and it is mapped to 10q24.1. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
Cite This Product
Anti-Fas Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00055)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
64%
Low homology
Cow
56%
Low homology
Human
56%
Low homology
Sheep
56%
Low homology
Monkey
55%
Low homology
Dog
49%
Low homology
Rabbit
41%
Low homology
Zebrafish
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Fas antibody
Flow Cytometry (FC) protocol for Fas antibody
Publications
Application:
Species:
Decreasing S1PR3 Alleviated Renal Tubular Lipid Deposition in Diabetic Nephropathy via AMPK/PPAR-γ/GPAT3 Pathway
Published: 2026-06-10
Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Application: WB
Impact Factor: 3.50
Species: Rat
PMID: 42268342
Understanding the relationship between inflammation, apoptosis, and diabetes osteoporosis: A bioinformatics approach and experimental verification
Published: 2024-09-28
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 39340228
Regulatory role of oxidative stress in retrorsine – Induced apoptosis and autophagy in primary rat hepatocytes
Published: 2024-05-28
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.20
Species: Rat
PMID: 38810283
Perfluorooctanoic acid exposure in early pregnancy induces oxidative stress in mice uterus and liver
Published: 2021-07-31
Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Application: IHC
Impact Factor: 4.22
Species: Mouse
PMID: 34331232
Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation
Published: 2008-10-27
Journal: CELL BIOCHEMISTRY AND FUNCTION
Application: IF
Impact Factor: 1.56
Species: Rat
PMID: 18956479
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Product Promise
We promise Anti-Fas Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00055
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026