|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-TIMP3 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 3(TIMP3) detection. Tested with WB, ELISA in Human;Mouse;Rat.|
|Cite This Product||Anti-TIMP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1077)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP3(198-211aa WAPPDKSIINATDP), different from the related rat and mouse sequences by one amino acid.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-TIMP3 antibody, PA1077, Western blotting
Lane 1: Recombinant Human TIMP3 Protein 10ng
Lane 2: Recombinant Human TIMP3 Protein 5ng
Lane 3: Recombinant Human TIMP3 Protein 2.5ng
Protein Target Info (Source: Uniprot.org)
|Protein Name||Metalloproteinase inhibitor 3|
|Alternative Names||Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3;|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix.|
|Molecular Weight||24145 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.|
|Research Areas||Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Neuroscience, Protease Inhibitors, Proteolysis / Ubiquitin, Sensory System, Signal Transduction, Visual System
*You can search these to find other products in these research areas.
|Background||Metalloproteinase inhibitor 3 is a protein that in humans is encoded by the TIMP3 gene. It is mapped to 22q12.1-q13.2. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of theextracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,