|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-TIMP-3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 3(TIMP3) detection. Tested with WB, ELISA in Human;Mouse;Rat.|
|Cite This Product||Anti-TIMP-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1077)|
|Replacement Item||This antibody may replace the following items: sc-27286|sc-30075|sc-373839|sc-373842|sc-6836|sc-9906 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP3(198-211aa WAPPDKSIINATDP), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Metalloproteinase inhibitor 3|
|Molecular Weight||24145 MW|
|Protein Function||Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.|
|Sequence Similarities||Belongs to the protease inhibitor I35 (TIMP) family.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix.|
|Alternative Names||Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3;|
|Research Areas|||cell biology|apoptosis|extracellular signals|granzymes| cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|timp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|mmp inhibitors| neuroscience|sensory system|visual system| cancer|invasion/microenvironment|timps| cell biology|proteolysis / ubiquitin|protease inhibitors|metalloprotease inhibitors||
Background for Metalloproteinase inhibitor 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TIMP-3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
ELISA , 0.1-0.5μg/ml, Human, -|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TIMP-3 Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human TIMP3 Protein 10ng
Lane 2: Recombinant Human TIMP3 Protein 5ng
Lane 3: Recombinant Human TIMP3 Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,