Human TIMP-3 ELISA Kit PicoKine®

TIMP-3 ELISA kit for Human

Human TIMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0523
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Product Name

Human TIMP-3 ELISA Kit PicoKine®

View all TIMP-3 ELISA kits

SKU/Catalog Number

EK0523

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TIMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TIMP-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0523)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: C24-P211

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0523 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0523 is reactive to TIMP3 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0523 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIMP-3

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Kit Components

Catalog Number Description Quantity
EK0523-CAP Anti-Human TIMP3 Pre-coated 96-well strip microplate 1
EK0523-ST Human TIMP3 Standard 2 vials, 10 ng/tube
EK0523-DA Human TIMP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0790.1510.1730.2500.4330.7461.4592.068

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TIMP-3, Dilution ratio of 1:1, concentration in serum and plasma is 600-1200 pg/ml..

Some articles we found to cite concentrations of TIMP-3 in samples: 25449074 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TIMP-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2331357438324814744049
Standard deviation18.2192.27271.7421.08120.86327.96
CV (%)7%6.8%6.2%8.5%8.2%8.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIMP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123326026124324911.794.7%
Sample 21357134213731251133047.333.5%
Sample 343833655422043204144288.526.9%
*number of samples for each test n=16.

Gene/Protein Information For TIMP3 (Source: Uniprot.Org, NCBI)

Gene Name

TIMP3

Full Name

Metalloproteinase inhibitor 3

Weight

24.145kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory); SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3 TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP3, check out the TIMP3 Infographic

TIMP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0523 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fibroblasts in Nodular Sclerosing Classical Hodgkin Lymphoma Are Defined by a Specific Phenotype and Protect Tumor Cells from Brentuximab-Vedotin Induced Injury

Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro

Denkovskij, J., Bagdonas, E., Kusleviciute, I., Mackiewicz, Z., Unguryte, A., Porvaneckas, N.,..., & Bernotiene, E. (2017). Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases an...

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4 Customer Q&As for Human TIMP-3 ELISA Kit PicoKine®

Question

Q: how to proceed with the analysis of ELISA data? I measured TIMP-3 level in plasma.

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-14

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TIMP-3 using the EK0523 Human TIMP-3 Picokine® ELISA Kit?

A. Rodriguez

Verified customer

Asked: 2019-11-16

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-11-16

Question

What is the composition of the diluent buffer for EK0523 and does it have any detergent in it?

Verified customer

Asked: 2019-06-14

Answer

Our diluent buffers for the Human TIMP-3 ELISA Kit PicoKine™ (EK0523) contains Sodium Azide, BSA, Tris, and NaCl. The ABC diluent buffer also contains TWEEN.

Boster Scientific Support

Answered: 2019-06-14

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

A. Zhao

Verified customer

Asked: 2015-11-20

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2015-11-20

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