Human TIMP-3 ELISA Kit PicoKine®

Product Name

Human TIMP-3 ELISA Kit PicoKine®

SKU/Catalog Number

EK0523

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

TIMP3 is an extracellular matrix-associated tissue inhibitor of metalloproteinases that regulates ECM remodeling by inhibiting multiple MMPs (and is broadly discussed in protease–inhibitor balance and shedding/remodeling contexts). Assay context: quantitative sandwich ELISA for Human TIMP-3 in serum, plasma, cell lysates, cell culture supernatants, and saliva; sensitivity <2 pg/mL; detection range 156–10,000 pg/mL. Commonly paired with matrix protease readouts such as MMP3 and MMP9 to interpret net remodeling potential (putative co-panel; sample-type dependent).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TIMP-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0523)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: C24-P211

Capture/Detection Antibodies

The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0523 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0523 is reactive to TIMP3 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0523 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIMP3

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Kit Components

Catalog Number	Description	Quantity
EK0523-CAP	Anti-Human TIMP3 Pre-coated 96-well strip microplate	1
EK0523-ST	Human TIMP3 Standard	2 vials, 10 ng/tube
EK0523-DA	Human TIMP3 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.029	0.144	0.276	0.518	0.876	1.409	1.924	2.059

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TIMP3, Dilution ratio of 1:1, concentration in serum and plasma is 600-1200 pg/ml..

Some articles we found to cite concentrations of TIMP3 in samples: 25449074 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TIMP-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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