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Product Info Summary
| SKU: | M08373 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-TIPRL Mouse Monoclonal Antibody [Clone ID: OTI3D11]
SKU/Catalog Number
M08373
Size
100 µl
Description
Boster Bio Anti-TIPRL mouse monoclonal antibody, clone OTI3D11 (formerly 3D11). Catalog# M08373. Tested in FC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-TIPRL Mouse Monoclonal Antibody [Clone ID: OTI3D11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08373)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3D11
Isotype
IgG1
Immunogen
Full length human recombinant protein of human TIPRL (NP_690866) produced in HEK293T cell.
Reactive Species
M08373 is reactive to TIPRL in Human, Mouse, Rat
Calculated molecular weight
31.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08373 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPRL.
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Flow cytometric analysis of Hela cells
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Flow cytometric analysis of Jurkat cells
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT) and TIPRL-Knockout HEK293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TIPRL monoclonal antibody M08373 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-TIPRL Mouse Monoclonal Antibody [Clone ID: OTI3D11] (M08373)
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