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Product Info Summary
| SKU: | A07983 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BAMBI Antibody
SKU/Catalog Number
A07983
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BAMBI Antibody (Catalog # A07983). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BAMBI antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-BAMBI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07983)
Host
Rabbit
Contents
BAMBI antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BAMBI antibody was raised against a 17 amino acid peptide near the center of human BAMBI. The immunogen is located within amino acids 100 - 150 of BAMBI.
Cross-reactivity
BAMBI antibody is human, mouse and rat reactive.
Reactive Species
A07983 is reactive to BAMBI in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.1 kDa
Background of BAMBI
BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07983 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BAMBI in mouse lung tissue lysate with BAMBI antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of BAMBI in human lung tissue with BAMBI antibody at 5 μg/ml.
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Immunofluorescence of BAMBI in human lung tissue with BAMBI antibody at 20 μg/ml.
Specific Publications For Anti-BAMBI Antibody (A07983)
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