This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Ddit3 antibody (A00311-4) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
Filter:
Flow Cytometry analysis of C6 cells using anti-Ddit3 antibody (A00311-4).
Overlay histogram showing C6 cells stained with A00311-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ddit3 Antibody (A00311-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing C6 cells stained with A00311-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ddit3 Antibody (A00311-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-Ddit3 Antibody Picoband®
Ddit3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Ddit3 antibody was positively detected by WB in:
mouse RAW2647 whole cell, rat C6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ddit3 antibody was positively detected by FC in:
C6 cell, RAW2647 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
ELISA
Mouse
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Human
Predicted
Predicted
Product Information
Product Name
Anti-Ddit3 Antibody Picoband®
View all DDIT3 Antibodies
View all DDIT3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Ddit3 recombinant protein (Position: E17-A168).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated Molecular Weight
19.2 kDa
Background of Ddit3
DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotictranscription factor that is encoded by the DDIT3 gene. It is mapped to 12q13.3. This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Cite This Product
Anti-Ddit3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00311-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
97%
High Homology
Monkey
91%
High Homology
Human
90%
High Homology
Cow
89%
Moderate Homology
Sheep
89%
Moderate Homology
Rabbit
88%
Moderate Homology
Dog
88%
Moderate Homology
Zebrafish
49%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Ddit3 antibody
Flow Cytometry (FC) protocol for Ddit3 antibody
Publications
Application:
Species:
BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress
Published: 2024-07-07
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38973202
Liproxstatin-1 attenuates acute hypertriglyceridemic pancreatitis through inhibiting ferroptosis in rats
Published: 2024-04-25
Journal: Scientific Reports
Application: WB
Impact Factor: 3.80
Species: Rat
PMID: 38664508
MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B
Published: 2020-04-26
Journal: Aging-US
Application: WB
Impact Factor: 4.83
Species: Mouse
PMID: 32335541
Inhibition of transient receptor potential melastatin 7 (TRPM7) channel induces RA FLSs apoptosis through endoplasmic reticulum (ER) stress
Published: 2014-04-15
Journal: CLINICAL RHEUMATOLOGY
Application: WB
Impact Factor: 1.77
Species: Rat
PMID: 24733250
Endoplasmic reticulum stress in diethylnitrosamine‑induced rat liver cancer
Published: 2013-10-29
Journal: Oncology Letters
Application: WB
Impact Factor: 0.24
Species: Rat
PMID: 24348814
Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect
Published: 2011-05-23
Journal: PLoS One
Application: WB
Impact Factor: 4.41
Species: Rat
PMID: 21625440
Review
Have you used Anti-Ddit3 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Ddit3 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00311-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026