Product Info Summary
| SKU: | A00362-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TMS1 PYCARD Antibody
SKU/Catalog Number
A00362-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TMS1 PYCARD Antibody catalog # A00362-2. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMS1 PYCARD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00362-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide of human ASC/TMS1(NP_037390.2).
Reactive Species
A00362-2 is reactive to PYCARD in Human, Mouse, Rat
Calculated molecular weight
21.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00362-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts of Mouse spleen, using ASC/TMS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Click image to see more details
Western blot analysis of extracts of Rat spleen, using ASC/TMS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Click image to see more details
Immunohistochemistry of paraffin-embedded human colon using ASC/TMS1 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Click image to see more details
Immunohistochemistry of paraffin-embedded human tonsil using ASC/TMS1 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Specific Publications For Anti-TMS1 PYCARD Antibody (A00362-2)
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