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NLRP3 antibody (A00034-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 42 Publications

a00034 2 nlrp3 primary antibodies fcm testing 2

Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (A00034-2).
Overlay histogram showing THP-1 cells stained with A00034-2 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (A00034-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control."

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Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®

NLRP3 antibody

42 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, WB

Tested Applications

NLRP3 antibody was positively detected by WB in:

human Hela whole cell, human U-87MG whole cell, rat spleen tissue, rat PC-12 whole cell, mouse thymus tissue, mouse lung tissue, mouse spleen tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

NLRP3 antibody was positively detected by FC in:

THP-1 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®
View all NLRP3 Antibodies

Form

Lyophilized

Uniprot

Q96P20

Gene ID

114548

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CIAS1/NALP3/NLRP3 recombinant protein (Position: D812-S1035).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

118 kDa

Calculated Molecular Weight

118.2 kDa

Background of NLRP3

NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3), also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 (NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD (ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Cite This Product

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00034-2)

Protocols

Western Blot protocol for NLRP3 antibody

Review Protocol

Flow Cytometry (FC) protocol for NLRP3 antibody

Review Protocol

Publications

Paeoniflorin alleviates CKD-associated constipation by modulating TPH1/AHR-related signaling and suppressing NLRP3/GSDMD-mediated pyroptosis

Published: 2026-07-10

Journal: Frontiers in Pharmacology

Application: WB

Impact Factor: 5.40

Species: Mouse

PMID: 42499504

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Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®

NLRP3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Paeoniflorin alleviates CKD-associated constipation by modulating TPH1/AHR-related signaling and suppressing NLRP3/GSDMD-mediated pyroptosis

GDF11 regulates hypoxia-induced pulmonary endothelial cell pyroptosis through SOX2/NLRP3 axis

Shrimp Oil Enhances Cognition by Suppressing Neuroinflammation in a Chronic Cerebral Hypoperfusion Rat Model

Dihydromyricetin: A potential candidate for the treatment of cholestatic liver injury via inhibition of the NLRP3 inflammasome

PLGA nanoparticles encapsulating fibroblast growth factor 21 alleviate acetaminophen-induced acute liver injury

Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor

Unveiling the gut-vascular connection: Gut microbiota regulates cardiovascular toxicity induced by combined exposure to PM2.5 and high-fat diet via butyric acid/NLRP3 pathway

Mechanisms of Dangguiliuhuang Tang in Streptozotocin-Induced Type 2 Diabetes Mellitus Mice Based on Network Pharmacology, Gut Microbiota, and Experimental Validation

An Integrated Approach of experiment validation and Single-cell RNA sequencing reveal dual-pathway inhibition of NLRP3/ferroptosis by flavonoids of Sanyeqing fibrous root in acute lung injury

Astragaloside IV Regulates the IRF7/NLRP3 Axis to Inhibit Neutrophil Extracellular Trap Formation and Alleviate Coxsackievirus B3-Induced Myocarditis

Neuroinflammation in hypothyroidism? Chrysin treatment as a strategy to reduce neuroinflammation induced by hypothyroidism

Berberine attenuates the expression of NLRP3 and downstream inflammasome effectors in diabetic retinopathy

Huanglian Jiedu Decoction improves the"central-peripheral"inflammatory microenvironment and enhances the cognitive function of APP/PS1 mice by inhibiting the activation of NLRP3 inflammasome mediated by gut microbiota

Red blood cells undergo lytic programmed cell death involving NLRP3

Therapeutic role of Prunella vulgaris L. polysaccharides in non-alcoholic steatohepatitis and gut dysbiosis

Paeonol inhibits NETs-mediated foam cell inflammation through the CitH3/NLRP3/caspase-1 signaling pathway in atherosclerosis

Acupuncture protects against ischemic stroke by inhibiting the NF-κB pathway

NLRP3 Inflammasome Activation Is Involved in Geniposide-Induced Hepatotoxicity

Activation of Ferroptosis and NF-κB/NLRP3/MAPK Pathways in Methylmercury-Induced Hepatotoxicity

Microglial priming by IFN-γ involves STAT1-mediated activation of the NLRP3 inflammasome

Stress-induced NLRP3 inflammasome activation and myelin alterations in the hippocampus of PTSD rats

Echinatin alleviates inflammation and pyroptosis in hypoxic-ischemic brain damage by inhibiting TLR4/ NF-κB pathway

Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome

Carnosine ameliorates postoperative cognitive dysfunction of aged rats by limiting astrocytes pyroptosis

Dopamine promotes Klebsiella quasivariicola proliferation and inflammatory response in the presence of macrophages

Phoenixin 20 ameliorates pulmonary arterial hypertension via inhibiting inflammation and oxidative stress

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

Protective role of thymoquinone in hyperlipidemia-induced liver injury in LDL-R−/−mice

TLR4-NLRP3-GSDMD-Mediated Pyroptosis Plays an Important Role in Aggravated Liver Injury of CD38-/- Sepsis Mice

Inhibition of endotoxin-induced acute lung injury in rats by bone marrow–derived mesenchymal stem cells: Role of Nrf2/HO-1 signal axis in inhibition of NLRP3 activation

Circular RNA Calm4 Regulates Hypoxia-Induced Pulmonary Arterial Smooth Muscle Cells Pyroptosis via the Circ-Calm4/miR-124-3p/PDCD6 Axis

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats

GLI1-mediated pulmonary artery smooth muscle cell pyroptosis contributes to hypoxia-induced pulmonary hypertension

Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell

Hydrogen sulfide mitigates myocardial inflammation by inhibiting nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome activation in diabetic rats

Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway

MiR-135b protects cardiomyocytes from infarction through restraining the NLRP3/caspase-1/IL-1β pathway

Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier

Ac-YVAD-cmk improves neurological function by inhibiting caspase-1-mediated inflammatory response in the intracerebral hemorrhage of rats

Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways

Quercetin suppresses NLRP3 inflammasome activation in epithelial cells triggered by Escherichia coli O157:H7

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