Anti-Caspase-1(P20)/CASP1 Antibody


SKU PA1440-1
Size 100μg/vial
Reactivity Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-Caspase-1(P20)/CASP1 Antibody
SKU/Catalog Number PA1440-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Caspase-1(CASP1) detection. Tested with WB in Mouse;Rat.
Cite This Product Anti-Caspase-1(P20)/CASP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1440-1)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1(137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids.
Reactivity Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Anti-Caspase-1(P20) antibody, PA1440-1, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Spleen Tissue Lysate
Lane 5: Mouse Testis Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P29452
Gene Name Casp1
Protein Name Caspase-1
Tissue Specificity High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle.
Alternative Names Caspase-1;CASP-1;3.4.22.36;Interleukin-1 beta convertase;IL-1BC;Interleukin-1 beta-converting enzyme;ICE;IL-1 beta-converting enzyme;p45;Caspase-1 subunit p20;Caspase-1 subunit p10;Casp1;Il1bc;
Subcellular Localization Cytoplasm.
Molecular Weight 45640 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Research Areas Mouse, Rat

*You can search these to find other products in these research areas.
Background Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

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Polyclonal antibody for Caspase 1/CASP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Mouse. Caspase 1/CASP1 information: Molecular Weight: 45640 MW; Subcellular Localization: Cytoplasm; Tissue Specificity: High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle.
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In stock
Order Product
PA1440-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
Zhang D, Yan H, Hu Y, Zhuang Z, Yu Z, Hang C. J Stroke Cerebrovasc Dis. 2015 May;24(5):972-9. Doi: 10.1016/J.Jstrokecerebrovasdis.2014.12.019. Epub 2015 Mar 23. Increased Expression Of Nlrp3 Inflammasome In Wall Of Ruptured And Unruptured Human Ce...
Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, Wang X, Altaf A, Wang L, He D, Wang H, Qu P. Int J Mol Med. 2015 May;35(5):1179-88. Doi: 10.3892/Ijmm.2015.2129. Epub 2015 Mar 9. P2X7R Is Involved In The Progression Of Atherosclerosis By Promoting Nlr...
Zhao Y, Wu S, Wu J, Jia P, Gao S, Yan X, Wang Y. Cancer Biol Ther. 2011 Jan 1;11(1):95-107. Doi: 10.4161/Cbt.11.1.13960. Introduction Of Hypoxia-Targeting P53 Fusion Protein For The Selective Therapy Of Non-Small Cell Lung Cancer.
Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Shu Hf, Yu Sx, Zhang Cq, Liu Sy, Wu Kf, Zang Zl, Yang H, Zhou Sw, Yin Q. Brain Dev. 2013 Mar;35(3):252-60. Doi: 10.1016/J.Braindev.2012.04.007. Epub 2012 May 28. Expression Of Trpv1 In Cortical Lesions From Patients With Tuberous Sclerosis Complex...
Wang Xf, Qu Xq, Zhang Tt, Zhang Jf. Exp Ther Med. 2015 Apr;9(4):1283-1291. Epub 2015 Feb 6. Testosterone Suppresses Ventricular Remodeling And Improves Left Ventricular Function In Rats Following Myocardial Infarction.
Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...
Wu Z, Wang L, Hou S. Neural Regen Res. 2012 Jul 5;7(19):1493-7. Doi: 10.3969/J.Issn.1673-5374.2012.19.008. Alpha B-Crystallin Improved Survival Of Retinal Ganglion Cells In A Rat Model Of Acute Ocular Hypertension.
Chen A, Dang T, Wang S, Tang N, Liu Y, Wu W. Sci China Life Sci. 2014 Jul;57(7):698-709. Doi: 10.1007/S11427-014-4680-8. Epub 2014 Jun 16. The In Vitro And In Vivo Anti-Tumor Effects Of Mtx-Fe3O 4-Plla-Peg-Plla Microspheres Prepared By Suspension-...
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.
Chen S, Peng H, Rowat A, Gao F, Zhang Z, Wang P, Zhang W, Wang X, Qu L. Brain Res. 2015 Aug 27;1618:205-11. Doi: 10.1016/J.Brainres.2015.05.027. Epub 2015 May 30. The Effect Of Concentration And Duration Of Normobaric Oxygen In Reducing Caspase-3 ...
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
Ji J, Yan X, Li Z, Lai Z, Liu J. Biomed Pharmacother. 2015 Feb;69:311-6. Doi: 10.1016/J.Biopha.2014.12.020. Epub 2014 Dec 19. Therapeutic Effects Of Intrathecal Versus Intravenous Monosialoganglioside Against Bupivacaine-Induced Spinal Neurotoxici...
Lin S, Lin K, Li W, Zhou X, Huang T. Endocrine. 2010 Jun;37(3):460-6. Doi: 10.1007/S12020-010-9337-1. Epub 2010 Apr 21. Maternal Diabetes Increases Apoptosis In Mice Oocytes, Not 2-Cell Embryos.
Li Wz, Li Wp, Huang Dk, Kan Hw, Wang X, Wu Wy, Yin Yy, Yao Yy. Behav Brain Res. 2012 Feb 1;227(1):142-9. Doi: 10.1016/J.Bbr.2011.10.038. Epub 2011 Oct 29. Dexamethasone And A????????-?????? Accelerate Learning And Memory Impairments Due To Elevate...
Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...
Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
Huang Kf, Huang Xp, Xiao Gq, Yang Hy, Lin Js, Diao Y. Biomed Pharmacother. 2014 May;68(4):455-61. Doi: 10.1016/J.Biopha.2014.03.005. Epub 2014 Mar 18. Kallistatin, A Novel Anti-Angiogenesis Agent, Inhibits Angiogenesis Via Inhibition Of The Nf-??b...
Fu Y, Zhao Z, Wu Y, Wu K, Xu X, Liu Y, Tong C. J Ethnopharmacol. 2012 Jan 6;139(1):26-33. Doi: 10.1016/J.Jep.2011.08.077. Epub 2011 Nov 10. Therapeutic Mechanisms Of Tongmai Dasheng Tablet On Tripterygium Glycosides Induced Rat Model For Premature...

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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