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Product Info Summary
| SKU: | PA1440-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Caspase-1(P20)/CASP1 Antibody Picoband®
SKU/Catalog Number
PA1440-1
BA2220 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-1 (P20)/CASP1 Antibody catalog # PA1440-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-1(P20)/CASP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1440-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1, different from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1440-1 is reactive to Casp1 in Mouse, Rat
Observed Molecular Weight
46 kDa
Calculated molecular weight
45.6 kDa
Background of Casp1
Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1440-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat thymus tissue, rat C6 whole cell, mouse thymus tissue
Validation Images & Assay Conditions
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Western blot analysis of CASP1 using anti-CASP1 antibody (PA1440-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 antigen affinity purified polyclonal antibody (Catalog # PA1440-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP1 at approximately 46 kDa. The expected band size for CASP1 is at 46 kDa.
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The application of SR9009 for 7 days reduced the expression of NLRP3 inflammasome-related proteins and inflammatory cytokines in the hippocampus after SE. a The expression of NLRP3, ASC, Caspase1, IL-1β, IL-18, IL-6, and TNF-α was detected by western blotting assay. b Densitometric analysis results of NLRP3, ASC, Caspase1, IL-1β, IL-18, IL-6, and TNF-α in the four groups. n = 5 for each group. The data presented as the mean ± SD. NS, not significant, * p < 0.05, † p < 0.001
Index in PubMed under a CC BY license. PMID: 32005256
Specific Publications For Anti-Caspase-1(P20)/CASP1 Antibody Picoband® (PA1440-1)
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17 Customer Q&As for Anti-Caspase-1(P20)/CASP1 Antibody Picoband®
Question
We are interested in using your anti-Caspase-1(P20)/CASP1 antibody for interleukin-1 beta production studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
We appreciate your inquiry. This PA1440-1 anti-Caspase-1(P20)/CASP1 antibody is validated on rat spleen tissue, brain tissue, tissue lysate, mouse spleen tissue, testis tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-10
Question
Will anti-Caspase-1(P20)/CASP1 antibody PA1440-1 work on feline WB with macrophage?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Our lab technicians have not tested anti-Caspase-1(P20)/CASP1 antibody PA1440-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Caspase-1(P20)/CASP1 antibody PA1440-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline macrophage in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-09
Question
Is a blocking peptide available for product anti-Caspase-1(P20)/CASP1 antibody (PA1440-1)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We do provide the blocking peptide for product anti-Caspase-1(P20)/CASP1 antibody (PA1440-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-27
Question
I see that the anti-Caspase-1(P20)/CASP1 antibody PA1440-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-09
Question
Has PA1440-1 been tested with IHC-F application?
Verified customer
Asked: 2019-03-29
Answer
We haven't got available appropriate samples for IHC-F test using the Anti-Caspase-1(P20)/CASP1 Antibody (PA1440-1). IHC-P result was not good.
Boster Scientific Support
Answered: 2019-03-29
Question
I was wanting to use your anti-Caspase-1(P20)/CASP1 antibody for WB for rat macrophage on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat macrophage identification?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
As indicated on the product datasheet, PA1440-1 anti-Caspase-1(P20)/CASP1 antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-28
Question
We are interested in to test anti-Caspase-1(P20)/CASP1 antibody PA1440-1 on rat macrophage for research purposes, then I may be interested in using anti-Caspase-1(P20)/CASP1 antibody PA1440-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
The products we sell, including anti-Caspase-1(P20)/CASP1 antibody PA1440-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-22
Question
I have a question about product PA1440-1, anti-Caspase-1(P20)/CASP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1440-1 anti-Caspase-1(P20)/CASP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-03
Question
I have attached the WB image, lot number and protocol we used for macrophage using anti-Caspase-1(P20)/CASP1 antibody PA1440-1. Please let me know if you require anything else.
N. Jackson
Verified customer
Asked: 2018-09-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-17
Question
We are currently using anti-Caspase-1(P20)/CASP1 antibody PA1440-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?
E. Rodriguez
Verified customer
Asked: 2018-07-11
Answer
The anti-Caspase-1(P20)/CASP1 antibody (PA1440-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-11
Question
Is there a BSA free version of anti-Caspase-1(P20)/CASP1 antibody PA1440-1 available?
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-1(P20)/CASP1 antibody PA1440-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-11
Question
Is this PA1440-1 anti-Caspase-1(P20)/CASP1 antibody reactive to the isotypes of CASP1?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
The immunogen of PA1440-1 anti-Caspase-1(P20)/CASP1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1(137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-01
Question
Will anti-Caspase-1(P20)/CASP1 antibody PA1440-1 work for WB with macrophage?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
According to the expression profile of macrophage, CASP1 is highly expressed in macrophage. So, it is likely that anti-Caspase-1(P20)/CASP1 antibody PA1440-1 will work for WB with macrophage.
Boster Scientific Support
Answered: 2018-04-26
Question
We have been able to see staining in rat leukocyte. Any tips? Is anti-Caspase-1(P20)/CASP1 antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
From literature leukocyte does express CASP1. From Uniprot.org, CASP1 is expressed in leukocyte, testis, macrophage, among other tissues. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-14
Question
Would PA1440-1 anti-Caspase-1(P20)/CASP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
You can see on the product datasheet, PA1440-1 anti-Caspase-1(P20)/CASP1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-26
Question
We were content with the WB result of your anti-Caspase-1(P20)/CASP1 antibody. However we have been able to see positive staining in testis cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP1 supposed to be expressed?
V. Mitchell
Verified customer
Asked: 2015-08-11
Answer
According to literature, testis does express CASP1. Generally CASP1 expresses in cytoplasm. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-08-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-Caspase-1(P20)/CASP1 antibody PA1440-1. Let me know if you need anything else.
J. Gonzalez
Verified customer
Asked: 2014-07-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-24
